Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris GS115 cells | Bispora sp. MEY-1 |
Protein Variants | Comment | Organism |
---|---|---|
D129K | the mutant shows increased catalytic efficiency towards trigalacturonic acid compared to the wild type enzyme | Bispora sp. MEY-1 |
D129K/D214N | inactive | Bispora sp. MEY-1 |
D129R | the mutant shows about wild type activity and stability | Bispora sp. MEY-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bispora sp. MEY-1 | A0A2R4K332 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | three glycosylation sites at Asn181, Asn201 and Asn282 | Bispora sp. MEY-1 |
Purification (Comment) | Organism |
---|---|
HiTrap Q Sepharose column chromatography | Bispora sp. MEY-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate + H2O | - |
Bispora sp. MEY-1 | D-(+)-galacturonate | - |
? | |
trigalacturonic acid + H2O | - |
Bispora sp. MEY-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37400, calculated from amino acid sequence | Bispora sp. MEY-1 |
? | x * 38000, deglycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
? | x * 50000, glycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
Synonyms | Comment | Organism |
---|---|---|
endo-PG | - |
Bispora sp. MEY-1 |
endo-polygalacturonase | - |
Bispora sp. MEY-1 |
PG28A | - |
Bispora sp. MEY-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bispora sp. MEY-1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme shows more than 60% activity between 60 and 80°C | Bispora sp. MEY-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme retains more than 90% activity after 30 min at 60°C | Bispora sp. MEY-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Bispora sp. MEY-1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 4.5 | the enzyme shows more than 60% activity between pH 2.5 and 4.5 | Bispora sp. MEY-1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.2 | 7 | the enzyme retains more than 90% activity after 1 h at pH 2.2-7.0 and 37°C | Bispora sp. MEY-1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bispora sp. MEY-1 | calculated from amino acid sequence | - |
4.3 |