Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.15 extracted from

  • Li, Y.; Wang, Y.; Tu, T.; Zhang, D.; Ma, R.; You, S.; Wang, X.; Yao, B.; Luo, H.; Xu, B.
    Two acidic, thermophilic GH28 polygalacturonases from Talaromyces leycettanus JCM 12802 with application potentials for grape juice clarification (2017), Food Chem., 237, 997-1003 .
    View publication on PubMed
Search for more literature for 3.2.1.15

Application

Application Comment Organism
food industry the enzyme is used for grape juice clarification Evansstolkia leycettana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Evansstolkia leycettana

Organism

Organism UniProt Comment Textmining
Evansstolkia leycettana A0A221LFZ6
-
-
Evansstolkia leycettana JCM 12802 A0A221LFZ6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has seven putative N-glycosylation sites Evansstolkia leycettana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin + H2O 20-40% esterified pectin, about 80% activity compared to polygalacturonic acid Evansstolkia leycettana ?
-
 ?
citrus pectin + H2O 50-70% esterified pectin, about 50% activity compared to polygalacturonic acid Evansstolkia leycettana ?
-
 ?
citrus pectin + H2O more than 85% esterified pectin, about 5% activity compared to polygalacturonic acid Evansstolkia leycettana ?
-
 ?
citrus pectin + H2O 20-40% esterified pectin, about 80% activity compared to polygalacturonic acid Evansstolkia leycettana JCM 12802 ?
-
 ?
citrus pectin + H2O 50-70% esterified pectin, about 50% activity compared to polygalacturonic acid Evansstolkia leycettana JCM 12802 ?
-
 ?
citrus pectin + H2O more than 85% esterified pectin, about 5% activity compared to polygalacturonic acid Evansstolkia leycettana JCM 12802 ?
-
 ?
polygalacturonic acid + H2O 100% activity Evansstolkia leycettana D-(+)-galacturonic acid + ?
-
 ?
polygalacturonic acid + H2O 100% activity Evansstolkia leycettana JCM 12802 D-(+)-galacturonic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Evansstolkia leycettana
? x * 37600, calculated from amino acid sequence Evansstolkia leycettana

Synonyms

Synonyms Comment Organism
endo-TePG28b
-
 Evansstolkia leycettana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Evansstolkia leycettana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 about 40% activity at 50°C, about 75% activity at 60°C, 100% activity at 70°C, about 60% activity at 80°C, less than 10% activity at 40°C or 90°C Evansstolkia leycettana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme retains about 70%, 40% and 10% activity after 1 h at 60°C, 70°C and 80°C, respectively Evansstolkia leycettana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Evansstolkia leycettana

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 about 35% activity at pH 2.5, about 70% activity at pH 3.0, 100% activity at pH 3.5, about 93% activity at pH 4.0, about 90% activity at pH 4.5, about 30% activity at pH 5.0 Evansstolkia leycettana

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 8 the enzyme retains about 80% activity after 1 h incubation at pH 2.0-8.0 Evansstolkia leycettana

pI Value

Organism Comment pI Value Maximum pI Value
Evansstolkia leycettana calculated from amino acid sequence
-
 5.1