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Literature summary for 3.2.1.15 extracted from
- Secretory expression and characterization of an acidic endo-polygalacturonase gene from Aspergillus niger SC323 in Saccharomyces cerevisiae (2015), J. Microbiol. Biotechnol., 25, 999-1006 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain EBY100 | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | about 92% residual activity at 1 mM | Aspergillus niger |  |
| Co2+ | about 75% residual activity at 1 mM | Aspergillus niger | |
| Mn2+ | about 55% residual activity at 1 mM | Aspergillus niger | |
| Ni2+ | about 82% residual activity at 1 mM | Aspergillus niger | |
| Pb2+ | about 38% residual activity at 1 mM | Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 125% activity at 1 mM | Aspergillus niger | |
| Cu2+ | about 123% activity at 1 mM | Aspergillus niger | |
| Fe2+ | about 115% activity at 1 mM | Aspergillus niger | |
| K+ | about 115% activity at 1 mM | Aspergillus niger | |
| Li+ | about 110% activity at 1 mM | Aspergillus niger | |
| Mg2+ | about 120% activity at 1 mM | Aspergillus niger | |
| Na+ | about 120% activity at 1 mM | Aspergillus niger | |
| Zn2+ | about 115% activity at 1 mM | Aspergillus niger | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | A0A0B4UL66 | - |
- |
| Aspergillus niger SC323 | A0A0B4UL66 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and ENrich SEC 650 gel filtration | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Aspergillus niger | galacturonic acid + digalacturonic acid + trigalacturonic acid | - |
? | |
| pectin + H2O | - |
Aspergillus niger SC323 | galacturonic acid + digalacturonic acid + trigalacturonic acid | - |
? | |
| polygalacturonate + H2O | - |
Aspergillus niger | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | - |
Aspergillus niger SC323 | oligogalacturonates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Aspergillus niger |
| ? | x * 38800, calculated from amino acid sequence | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-pgaA | - |
Aspergillus niger |
| endo-polygalacturonase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | more than 60% activity between 30 and 60°C | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | the enzyme retains more than 50% of its activity after incubation at 35-70°C for 60 min, but no activity remains after incubation at 70°C for 2 h | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | more than 60% activity between pH 3.0 and 6.0 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | the enzyme shows good stability within the pH range of 3.0-6.0 for different incubation times and over 65% of activity is retained | Aspergillus niger |
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