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Literature summary for 3.2.1.159 extracted from

  • Suzuki, H.; Sawai, Y.; Suzuki, T.; Kawai, K.
    Purification and characterization of an extracellular alpha-neoagarooligosaccharide hydrolase from Bacillus sp. MK03 (2002), J. Biosci. Bioeng., 93, 456-463.
    View publication on PubMed
Search for more literature for 3.2.1.159

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 2 mM, 9% residual activity Bacillus sp. (in: Bacteria)
CuCl2 2 mM, 7% residual activity Bacillus sp. (in: Bacteria)
EDTA 2 mM, 47% residual activity Bacillus sp. (in: Bacteria)
HgCl2 2 mM, no residual activity Bacillus sp. (in: Bacteria)
NiCl2 2 mM, 10% residual activity Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate 0.2 mM 60% residual activity Bacillus sp. (in: Bacteria)
SDS 2 mM, 53% residual activity Bacillus sp. (in: Bacteria)
SrCl2 2 mM, 43% residual activity Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 2 mM, 163% of initial activity Bacillus sp. (in: Bacteria)
SnCl2 2 mM, 121% of initial activity Bacillus sp. (in: Bacteria)
ZnCl2 2 mM, 128% of initial activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 8 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)
320000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) MK03
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.2
-
 pH 7.0, 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-neoagarobiose + H2O 40% hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + D-galactose
-
 ?
alpha-1,3-neoagarobiose + H2O 40% hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + D-galactose
-
 ?
neoagarohexaose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + agaropentaose
-
 ?
neoagarohexaose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + agaropentaose
-
 ?
neoagarotetraose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + agarotriose
-
 ?
neoagarotetraose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + agarotriose
-
 ?

Subunits

Subunits Comment Organism
More N-terminal amino acid sequence Bacillus sp. (in: Bacteria)
octamer 8 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable up to, 30 min, 96% residual activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 8.6 stable within this range Bacillus sp. (in: Bacteria)