Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Photobacterium profundum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
1.2 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
1.4 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
2.5 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
4.5 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
4.7 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum | |
17.5 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
18.8 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
28.6 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photobacterium profundum | - |
- |
- |
Photobacterium profundum SS9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Photobacterium profundum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
crude extract, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C | Photobacterium profundum |
0.046 | - |
purified enzyme, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C | Photobacterium profundum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucosaminide + H2O | - |
Photobacterium profundum | 4-nitrophenol + beta-D-glucosamine | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Photobacterium profundum | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Photobacterium profundum SS9 | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Photobacterium profundum | ? | - |
? | |
cellobiose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
cellopentaose + H2O | - |
Photobacterium profundum | ? | - |
? | |
cellopentaose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
cellotetraose + H2O | - |
Photobacterium profundum | ? | - |
? | |
cellotriose + H2O | - |
Photobacterium profundum | ? | - |
? | |
cellotriose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
chitobiose + H2O | - |
Photobacterium profundum | D-glucosamine | - |
? | |
deacetylated chitosan + H2O | slight hydrolysis | Photobacterium profundum | ? | - |
? | |
GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
additional information | the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose | Photobacterium profundum | ? | - |
? | |
additional information | the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose | Photobacterium profundum SS9 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-D-glucosaminidase | - |
Photobacterium profundum |
PBPRA0520 protein | - |
Photobacterium profundum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in 50 mM phosphate buffer at pH 7.0 | Photobacterium profundum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
0.19 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
47.7 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
52 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
54.2 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
68.8 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum | |
77.4 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
97.6 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
152.9 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at 37°C | Photobacterium profundum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Photobacterium profundum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
0.126 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
2.4 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum | |
2.5 | - |
cellopentaose | pH 7.0, 37°C | Photobacterium profundum | |
2.7 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
2.9 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
31.3 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
32.6 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum | |
41.9 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
69.2 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum |