Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Gallus gallus |
Crystallization (Comment) | Organism |
---|---|
mutants K33A and K33N. The side chain of K33 in wild-type hydrogen bonds with N37 involved in the substrate-binding region. Orientation of N37 differs in mutants K33A and K33N | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
K33A | 140% of wild-type lytic activity, 116% of wild-type activity on glycol chitin | Gallus gallus |
K33N | 130% of wild-type lytic activity, 111% of wild-type activity on glycol chitin | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P00698 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycol chitin + H2O | - |
Gallus gallus | ? | - |
? | |
additional information | measurement of activity by lytic activity against Micrococcus luteus | Gallus gallus | ? | - |
? |