Inhibitors | Comment | Organism | Structure |
---|---|---|---|
c-type inhibitor Ivy | complete inhibition | Salmo salar | |
Ca2+ | 34% activation at 5 mM, 150% activation at 10 mM, 280% at 5 mM of recombinant enzyme. 58% Inhibition at 10 mM, 97% at 50 mM of native enzyme, 66% inhibition at 20 mM, 97% at 50 mM of recombinant enzyme; 87% inhibition at 5 mM, 93% at 10 mM | Salmo salar | |
g-type inhibitor PliG | complete inhibition | Salmo salar | |
additional information | no inhibition by c-type inhibitor Ivy; no inhibition by g-type inhibitor PliG | Salmo salar | |
Na+ | 14% inhibition at 10 mM, 81% at 100 mM; activates the native enzyme 3.43fold at 50 mM, the recombinant enzyme 4.3fold at 100 mM, inhibition of native, not recombinant, enzyme at 200 mM | Salmo salar |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 34% activation at 5 mM, 150% activation at 10 mM, 280% at 5 mM of recombinant enzyme. 58% Inhibition at 10 mM, 97% at 50 mM of native enzyme, 66% inhibition at 20 mM, 97% at 50 mM of recombinant enzyme | Salmo salar | |
Na+ | activates the native enzyme 3.43fold at 50 mM, the recombinant enzyme 4.3fold at 100 mM, inhibition of native, not recombinant, enzyme at 200 mM | Salmo salar |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmo salar | A6PZ97 | - |
- |
Salmo salar | B5XA65 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from head kidneys by ultrafiltration, cation exchange chromatography, ultrafiltration, and gel filtration | Salmo salar |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | contains exclusively g-type lysozyme | Salmo salar | - |
gill | contains exclusively g-type lysozyme | Salmo salar | - |
head kidney | contains c-type and g-type lysozymes | Salmo salar | - |
liver | contains exclusively g-type lysozyme | Salmo salar | - |
macrophage | contains c-type and g-type lysozymes | Salmo salar | - |
additional information | c-type lysozyme occurs only in head kidney and spleen | Salmo salar | - |
spleen | contains c-type and g-type lysozymes | Salmo salar | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12500 | - |
purified enzyme, pH 6.0, temperature not specified in the publication | Salmo salar |
18800 | - |
purified enzyme, pH 6.0, temperature not specified in the publication | Salmo salar |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lyophilized cell wall of Micrococcus luteus + H2O | - |
Salmo salar | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
c-type lysozyme | - |
Salmo salar |
chicken-type lysozyme | - |
Salmo salar |
g-type lysozyme | - |
Salmo salar |
Goose-type lysozyme | - |
Salmo salar |
n-SalC | - |
Salmo salar |
n-SalG | - |
Salmo salar |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | n-SalC lysozyme displays a sigmoidally increasing activity profile from 4°C to 70°C | Salmo salar |
22 | 60 | native g-type lysozyme activity slowly increases from a relatively high lytic activity at 4°C (45% of maximum) to peak lytic activity at 60°C. The recombinant enzyme reaches maximum activity at 22°C, high catalytic activity at 4°C and no activity at 60°C | Salmo salar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 6 | assay at | Salmo salar |
Organism | Comment | Expression |
---|---|---|
Salmo salar | real-time PCR analysis reveals that in macrophages g-type lysozyme shows a constitutive gene expression level independent of lipopolysaccharides | additional information |
Salmo salar | real-time PCR analysis reveals that the c-type lysozyme transcription in macrophages is highly inducible by lipopolysaccharide | up |
General Information | Comment | Organism |
---|---|---|
physiological function | lysozymes represent important innate immune components against bacteria | Salmo salar |