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Literature summary for 3.2.1.17 extracted from
- Purification and characterization of lysozyme from filipino venus, Ruditapes philippinarum (2012), Food Sci. Biotechnol., 21, 1463-1468.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ZnCl2 | inhibits at 2-30 mM | Ruditapes philippinarum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | activates at 2-5 mM | Ruditapes philippinarum | |
| MgCl2 | activates at 2-5 mM | Ruditapes philippinarum | |
| NaCl | activates at 10-70 mM | Ruditapes philippinarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13400 | - |
x * 13400, SDS-PAGE | Ruditapes philippinarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruditapes philippinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzme 3400fold by cation exchange chromatography and gel filtration | Ruditapes philippinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37600 | - |
purified native enzyme, substrate Micrococcus lysodeikticus cell walls, pH 6.0, 25°C | Ruditapes philippinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Micrococcus lysodeikticus cell wall + H2O | - |
Ruditapes philippinarum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 13400, SDS-PAGE | Ruditapes philippinarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Ruditapes philippinarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | activity range, profile overview | Ruditapes philippinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
purified enzyme, pH 6.0, 10-30 min, stable at | Ruditapes philippinarum |
| 40 | - |
purified enzyme, pH 6.0, 10-30 min, loss of 25-30% activity | Ruditapes philippinarum |
| 80 | - |
purified enzyme, pH 6.0, 10-30 min, loss of 45% activity | Ruditapes philippinarum |
| 100 | - |
purified enzyme, pH 6.0, loss of 60% activity after 10 min, inactivation after 30 min | Ruditapes philippinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Ruditapes philippinarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile overview | Ruditapes philippinarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | purified enzyme, 25°C, loss of 40% activity | Ruditapes philippinarum |
| 1 | 3 | purified enzyme, 25°C, inactivation | Ruditapes philippinarum |
| 8 | - |
purified enzyme, 25°C, completely stable up to | Ruditapes philippinarum |
| 11 | - |
purified enzyme, 25°C, loss of 20% activity | Ruditapes philippinarum |
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