Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.17 extracted from

  • Kurdyumov, A.S.; Manuvera, V.A.; Baskova, I.P.; Lazarev, V.N.
    A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein - Destabilase-Lysozyme from medicinal leech (2015), BMC Biochem., 16, 27 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Hirudo medicinalis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Hirudo medicinalis
NaCl almost complete inhibition at 0.8 M Hirudo medicinalis

Organism

Organism UniProt Comment Textmining
Hirudo medicinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal chelate affinity chromatography Hirudo medicinalis

Source Tissue

Source Tissue Comment Organism Textmining
salivary gland
-
 Hirudo medicinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall of Bacillus subtilis + H2O
-
 Hirudo medicinalis ?
-
 ?
cell wall of Escherichia coli + H2O
-
 Hirudo medicinalis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 16000 + x * 28000 + x * 44000, SDS-PAGE, recombinant isoform mlDL-Ds3 forms stable oligomeric complexes Hirudo medicinalis

Synonyms

Synonyms Comment Organism
destabilase-lysozyme
-
 Hirudo medicinalis
mlD-Ds1 isoform Hirudo medicinalis
mlD-Ds2 isoform Hirudo medicinalis
mlD-Ds3 isoform Hirudo medicinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Hirudo medicinalis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 highest activity between pH 5.0 and 9.0 Hirudo medicinalis