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Literature summary for 3.2.1.20 extracted from

  • Giblin, M.; Kelly, C.T.; Fogarty, W.M.
    Thermostable alpha-glucosidase produced by Bacillus caldovelox DSM411 (1987), Can. J. Microbiol., 33, 614-618.
No PubMed abstract available
Search for more literature for 3.2.1.20

Activating Compound

Activating Compound Comment Organism Structure
2,2'-dipyridyl 1 mM, about 6.5fold activation [Bacillus] caldovelox
D-glucose activates [Bacillus] caldovelox
EDTA 1 mM, about 4.5fold activation [Bacillus] caldovelox
p-phenanthroline 1 mM, about 8fold activation [Bacillus] caldovelox

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, activates [Bacillus] caldovelox
D-glucono-delta-lactone
-
 [Bacillus] caldovelox
phenyl-alpha-D-glucoside
-
 [Bacillus] caldovelox
ribose
-
 [Bacillus] caldovelox
Tris
-
 [Bacillus] caldovelox

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 [Bacillus] caldovelox 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, about 4.5fold activation [Bacillus] caldovelox
Sr2+ 10 mM, 3fold activation [Bacillus] caldovelox

Organism

Organism UniProt Comment Textmining
[Bacillus] caldovelox
-
 DSM411
-
[Bacillus] caldovelox DSM 411
-
 DSM411
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Bacillus] caldovelox

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
71.4
-
-
 [Bacillus] caldovelox

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O no activity [Bacillus] caldovelox 2 alpha-D-glucose
-
 ?
isomaltose + H2O no activity [Bacillus] caldovelox DSM 411 2 alpha-D-glucose
-
 ?
maltose + H2O
-
 [Bacillus] caldovelox alpha-D-glucose + D-glucose
-
 ?
maltose + H2O
-
 [Bacillus] caldovelox DSM 411 alpha-D-glucose + D-glucose
-
 ?
maltotriose + H2O
-
 [Bacillus] caldovelox maltose + D-glucose
-
 ?
maltotriose + H2O
-
 [Bacillus] caldovelox DSM 411 maltose + D-glucose
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O best substrate [Bacillus] caldovelox 4-nitrophenol + D-glucose
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O best substrate [Bacillus] caldovelox DSM 411 4-nitrophenol + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 [Bacillus] caldovelox

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 about 35% of maximal activity at 20°C and at 70°C [Bacillus] caldovelox

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable [Bacillus] caldovelox
70
-
 1 h, 50% loss of activity. Retains 83% or more activity after 1.5 h in presence of Mn2+, Cys, and His [Bacillus] caldovelox

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 [Bacillus] caldovelox

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 10% of maximal activity, pH 8.0: about 55% of maximal activity [Bacillus] caldovelox

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 20 min, retains more than 80% of its activity [Bacillus] caldovelox
5.5 6.5 20 min, completely stable [Bacillus] caldovelox