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Literature summary for 3.2.1.20 extracted from

  • Brizova, K.; Kralova, B.; Demnerova, K.
    Isolation and characterization of alpha-glucosidase from Aspergillus niger (1992), J. Chromatogr., 593, 125-131.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.87
-
maltose
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
x * 89000, glucoamylase, SDS-PAGE Aspergillus niger
131000
-
x * 131000, maltase, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Penicillium brevicompactum
-
-
-
Penicillium citrinum
-
-
-
Saccharomyces cerevisiae
-
type Malaga and type Palestina
-
Schizosaccharomyces pombe
-
-
-
Wickerhamomyces anomalus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Saccharomyces cerevisiae
-
culture medium
-
Aspergillus niger
-
culture medium
-
Schizosaccharomyces pombe
-
culture medium
-
Penicillium citrinum
-
culture medium
-
Wickerhamomyces anomalus
-
culture medium
-
Penicillium brevicompactum
-
liver
-
Aspergillus niger
-

Storage Stability

Storage Stability Organism
-20°C, 15 days, 41% loss of maltase activity, 21% loss of glucoamylase activity Aspergillus niger
4°C, 15 days, 21% loss of maltase activity, 46% loss of glucoamylase activity Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
Saccharomyces cerevisiae alpha-D-glucose + D-glucose
-
?
maltose + H2O
-
Aspergillus niger alpha-D-glucose + D-glucose
-
?
starch + H2O
-
Aspergillus niger alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 131000, maltase, SDS-PAGE Aspergillus niger
? x * 89000, glucoamylase, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
maltase activity Aspergillus niger
60
-
glucoamylase Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50 5°C: about 50% of maximal activity, 50°C: about 20% of maximal activity, maltase Aspergillus niger
5 80 5°C: about 50% of maximal activity, 80°C: about 40% of maximal activity, glucoamylase Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3 7 pH 3.0: about 30% of maximal activity, pH 7.0: about 80% of maximal activity Aspergillus niger