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Literature summary for 3.2.1.20 extracted from
- Production and properties of alpha-glucosidase from Lactobacillus acidophilus (1983), Appl. Environ. Microbiol., 46, 1380-1387.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | stimulates | Lactobacillus acidophilus |  |
| L-Cys | stimulates | Lactobacillus acidophilus | |
| L-His | stimulates | Lactobacillus acidophilus | |
| Tris | stimulates | Lactobacillus acidophilus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Iodoacetamide | - |
Lactobacillus acidophilus | |
| Ca2+ | - |
Lactobacillus acidophilus | |
| Co2+ | - |
Lactobacillus acidophilus | |
| Cu2+ | - |
Lactobacillus acidophilus | |
| Hg2+ | - |
Lactobacillus acidophilus | |
| Ni2+ | - |
Lactobacillus acidophilus | |
| Rose bengal | - |
Lactobacillus acidophilus | |
| Urea | 4.5 M, 22 min, 40% inhibition | Lactobacillus acidophilus | |
| Zn2+ | - |
Lactobacillus acidophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.5 | - |
4-nitrophenyl beta-D-glucopyranoside | - |
Lactobacillus acidophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Lactobacillus acidophilus | 5737 | - |
| intracellular | - |
Lactobacillus acidophilus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates | Lactobacillus acidophilus | |
| Mn2+ | stimulates | Lactobacillus acidophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus acidophilus | - |
IFO 3532 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus acidophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1451 | - |
- |
Lactobacillus acidophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Lactobacillus acidophilus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Lactobacillus acidophilus | 4-nitrophenol + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Lactobacillus acidophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 50% of maximal activity, 50°C: about 60% of maximal activity | Lactobacillus acidophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Lactobacillus acidophilus |
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