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Literature summary for 3.2.1.20 extracted from
- alpha-Glucosidase from grape berries: partial purification and characterization (1978), Planta, 142, 195-201.
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freezing and thawing | Vitis vinifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | - |
Vitis vinifera |  |
| fructose | - |
Vitis vinifera | |
| glycerol | competitive inhibitor | Vitis vinifera | |
| Hg2+ | 1 mM, complete inhibition | Vitis vinifera | |
| methyl-alpha-D-glucopyranoside | competitive | Vitis vinifera | |
| ribose | - |
Vitis vinifera | |
| sucrose | competitive | Vitis vinifera | |
| Tris | - |
Vitis vinifera | |
| turanose | competitive | Vitis vinifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Vitis vinifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vitis vinifera | - |
var. Riesling | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| maltotetraose + 3 H2O = 4 alpha-D-glucose | ping-pong mechanism | Vitis vinifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| berry | - |
Vitis vinifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 492 | - |
- |
Vitis vinifera |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 months | Vitis vinifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Vitis vinifera | 2 alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Vitis vinifera | alpha-D-glucose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Vitis vinifera | maltose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Vitis vinifera | 4-nitrophenol + D-glucose | - |
? | |
| starch + H2O | - |
Vitis vinifera | alpha-D-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
1 h, pH 5, stable | Vitis vinifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | - |
- |
Vitis vinifera |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
37°C, 1 h, stable | Vitis vinifera |
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