Any feedback?
Literature summary for 3.2.1.20 extracted from
- Purification and properties of extracellular alpha-glucosidase of a thermophile, Bacillus thermoglucosidius KP 1006 (1976), Biochim. Biophys. Acta, 445, 386-397.
General Stability
| General Stability | Organism |
|---|---|
| 45% ethanol, 7.2 M urea or 0.06% SDS, fail to inactivate the enzyme at 25°C. The tolerance is extremely reduced at 60°C | Parageobacillus thermoglucosidasius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Parageobacillus thermoglucosidasius |  |
| Ca2+ | - |
Parageobacillus thermoglucosidasius | |
| Co2+ | - |
Parageobacillus thermoglucosidasius | |
| Cu2+ | - |
Parageobacillus thermoglucosidasius | |
| D-glucono-delta-lactone | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| D-glucose | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| Fe3+ | - |
Parageobacillus thermoglucosidasius | |
| Hg2+ | - |
Parageobacillus thermoglucosidasius | |
| His | - |
Parageobacillus thermoglucosidasius | |
| isomaltose | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| Mg2+ | - |
Parageobacillus thermoglucosidasius | |
| Mn2+ | - |
Parageobacillus thermoglucosidasius | |
| p-nitrophenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| Pb2+ | - |
Parageobacillus thermoglucosidasius | |
| Tris | - |
Parageobacillus thermoglucosidasius | |
| Zn2+ | - |
Parageobacillus thermoglucosidasius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| 3 | - |
phenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| 3.2 | - |
isomaltose | - |
Parageobacillus thermoglucosidasius | |
| 37 | - |
methyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Parageobacillus thermoglucosidasius | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Parageobacillus thermoglucosidasius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parageobacillus thermoglucosidasius | - |
- |
- |
| Parageobacillus thermoglucosidasius KP 1006 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Parageobacillus thermoglucosidasius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 164 | - |
- |
Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Parageobacillus thermoglucosidasius | 2 alpha-D-glucose | - |
? | |
| isomaltose + H2O | - |
Parageobacillus thermoglucosidasius KP 1006 | 2 alpha-D-glucose | - |
? | |
| methyl-alpha-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius | methanol + alpha-D-glucose | - |
? | |
| methyl-alpha-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | methanol + alpha-D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Parageobacillus thermoglucosidasius | 4-nitrophenol + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | 4-nitrophenol + D-glucose | - |
? | |
| phenyl alpha-D-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius | phenol + alpha-D-glucose | - |
? | |
| phenyl alpha-D-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | phenol + alpha-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Parageobacillus thermoglucosidasius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 2 h, stable | Parageobacillus thermoglucosidasius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Parageobacillus thermoglucosidasius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | at 31°C, stable | Parageobacillus thermoglucosidasius |
| 6 | 8.5 | 55.5°C, stable | Parageobacillus thermoglucosidasius |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
