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Literature summary for 3.2.1.20 extracted from
- Localization and characterization of alpha-glucosidase activity in Lactobacillus brevis (1994), Appl. Environ. Microbiol., 60, 3074-3078.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | the enzyme together with the enzyme of Brettanomyces lambicus seems to be the key factor in the overattenuation of lambic beer | Levilactobacillus brevis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Tris | - |
Levilactobacillus brevis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Levilactobacillus brevis | |
| 3 | - |
maltose | - |
Levilactobacillus brevis | |
| 5.2 | - |
maltotriose | - |
Levilactobacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48500 | 53000 | gel filtration | Levilactobacillus brevis |
| 50000 | - |
gel filtration | Levilactobacillus brevis |
| 66000 | - |
1 * 66000, SDS-PAGE | Levilactobacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Levilactobacillus brevis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | - |
Levilactobacillus brevis | alpha-D-glucose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Levilactobacillus brevis | maltose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Levilactobacillus brevis | 4-nitrophenol + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Levilactobacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 30 min, stable | Levilactobacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Levilactobacillus brevis |
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Release 2023.1 (January 2023)
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