Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | competitive, inhibitory potential depends on the substrate used | Entamoeba histolytica | |
6-deoxy-D-glucose | competitive to 4-methylumbelliferyl alpha-D-glucoside | Entamoeba histolytica | |
australine | competitive, inhibitory potential depends on the substrate used | Entamoeba histolytica | |
castanospermine | competitive, inhibitory potential depends on the substrate used | Entamoeba histolytica | |
D-glucose | competitive to 4-methylumbelliferyl alpha-D-glucoside | Entamoeba histolytica | |
additional information | competition level between inhibitors and substrates, overview | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Entamoeba histolytica | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE | Entamoeba histolytica |
68000 | - |
2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE | Entamoeba histolytica |
243000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing | ? | - |
? | |
additional information | Entamoeba histolytica HM1:IMSS | the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Entamoeba histolytica HM1:IMSS | - |
- |
- |
Purification (Comment) | Organism |
---|---|
673fold by three chromatographical steps: ion exchange, gel filtration and adsorption chromatography | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.36 | - |
purified enzyme | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-glucoside + H2O | synthetic fluorogenic substrate, competitive to the other substrates | Entamoeba histolytica | 4-methylumbelliferol + alpha-D-glucose | - |
? | |
isomaltose + H2O | best substrate | Entamoeba histolytica | 2 alpha-D-glucose | - |
? | |
isomaltose + H2O | best substrate | Entamoeba histolytica HM1:IMSS | 2 alpha-D-glucose | - |
? | |
maltose + H2O | - |
Entamoeba histolytica | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Entamoeba histolytica HM1:IMSS | alpha-D-glucose + D-glucose | - |
? | |
additional information | the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing | Entamoeba histolytica | ? | - |
? | |
additional information | no activity with trehalose, kojibiose, and cellobiose | Entamoeba histolytica | ? | - |
? | |
additional information | the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
additional information | no activity with trehalose, kojibiose, and cellobiose | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
nigerose + H2O | - |
Entamoeba histolytica | 2 alpha-D-glucose | - |
? | |
nigerose + H2O | - |
Entamoeba histolytica HM1:IMSS | 2 alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE | Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
1-deoxynojirimycin | versus isomaltose, pH 5.5, 45°C | Entamoeba histolytica | |
0.0015 | - |
castanospermine | versus isomaltose, pH 5.5, 45°C | Entamoeba histolytica | |
0.22 | - |
6-deoxy-D-glucose | versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C | Entamoeba histolytica | |
0.23 | - |
D-glucose | versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C | Entamoeba histolytica |