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Literature summary for 3.2.1.20 extracted from
- Novel alpha-glucosidase from Aspergillus nidulans with strong transglycosylation activity (2002), Appl. Environ. Microbiol., 68, 1250-1256.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli, phylogenetic analysis | Aspergillus nidulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
maltotriose | pH 5.5, 45°C | Aspergillus nidulans |  |
| 0.51 | - |
maltose | pH 5.5, 45°C | Aspergillus nidulans | |
| 0.64 | - |
maltotetraose | pH 5.5, 45°C | Aspergillus nidulans | |
| 0.81 | - |
nigerose | pH 5.5, 45°C | Aspergillus nidulans | |
| 0.89 | - |
trehalose | pH 5.5, 45°C | Aspergillus nidulans | |
| 0.96 | - |
maltopentaose | pH 5.5, 45°C | Aspergillus nidulans | |
| 1.3 | - |
isomaltose | pH 5.5, 45°C | Aspergillus nidulans | |
| 1.3 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 45°C | Aspergillus nidulans | |
| 1.9 | - |
kojibiose | pH 5.5, 45°C | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
| 74000 | - |
1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
| 130000 | - |
gel filtration | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | Q9C1S7 | alpha-glucosidase B, i.e. AgdB | - |
| Aspergillus nidulans ABPU1 | Q9C1S7 | alpha-glucosidase B, i.e. AgdB | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus nidulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 52fold to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange and gel chromatography | Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
purified enzyme | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| isomaltose + H2O | - |
Aspergillus nidulans | 2 D-glucose | - |
? | |
| isomaltose + H2O | - |
Aspergillus nidulans ABPU1 | 2 D-glucose | - |
? | |
| kojibiose + H2O | - |
Aspergillus nidulans | alpha-D-glucose + D-glucose | - |
? | |
| kojibiose + H2O | - |
Aspergillus nidulans ABPU1 | alpha-D-glucose + D-glucose | - |
? | |
| maltopentaose + H2O | - |
Aspergillus nidulans | D-glucose + ? | - |
? | |
| maltose + H2O | - |
Aspergillus nidulans | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Aspergillus nidulans ABPU1 | 2 D-glucose | - |
? | |
| maltotetraose + H2O | - |
Aspergillus nidulans | maltotriose + D-glucose | - |
? | |
| maltotriose + H2O | best substrate | Aspergillus nidulans | maltose + D-glucose | - |
? | |
| additional information | the enzyme shows strong transglycosylation activity | Aspergillus nidulans | ? | - |
? | |
| additional information | the enzyme shows strong transglycosylation activity | Aspergillus nidulans ABPU1 | ? | - |
? | |
| nigerose + H2O | - |
Aspergillus nidulans | 2 alpha-D-glucose | - |
? | |
| nigerose + H2O | - |
Aspergillus nidulans ABPU1 | 2 alpha-D-glucose | - |
? | |
| trehalose + H2O | - |
Aspergillus nidulans | alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 74000 + 1 * 55000, SDS-PAGE | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgdB | - |
Aspergillus nidulans |
| alpha-glucosidase B | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
over 90% retained activity up to | Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | loss of less than 10% activity | Aspergillus nidulans |
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Release 2023.1 (January 2023)
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