KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | determination of subsite affinities, kinetics | Apis mellifera | |
2 | 3 | phenyl alpha-D-glucoside | pH 5.5, 35°C | Apis mellifera | |
10 | - |
maltotriose | pH 5.5, 35°C | Apis mellifera | |
11 | - |
maltose | pH 5.5, 35°C | Apis mellifera | |
13 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 35°C | Apis mellifera | |
16 | - |
maltotetraose | pH 5.5, 35°C | Apis mellifera | |
30 | - |
sucrose | pH 5.5, 35°C | Apis mellifera | |
31 | - |
turanose | pH 5.5, 35°C | Apis mellifera | |
44 | - |
nigerose | pH 5.5, 35°C | Apis mellifera | |
56 | - |
isomaltose | pH 5.5, 35°C | Apis mellifera | |
63 | - |
maltopentaose | pH 5.5, 35°C | Apis mellifera | |
63 | - |
maltohexaose | pH 5.5, 35°C | Apis mellifera | |
67 | - |
kojibiose | pH 5.5, 35°C | Apis mellifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
about, gel filtration | Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | - |
alpha-glucosidase III | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | about 7.4% carbohydrate content | Apis mellifera |
Purification (Comment) | Organism |
---|---|
to homogeneity, by salting-out chromatography and 5 additional chromatographic steps | Apis mellifera |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Apis mellifera |
11.3 | - |
purified enzyme | Apis mellifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | high activity | Apis mellifera | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | low activity | Apis mellifera | 2 alpha-D-glucose | - |
? | |
kojibiose + H2O | - |
Apis mellifera | alpha-D-glucose + D-glucose | - |
? | |
maltohexaose + H2O | low activity | Apis mellifera | maltopentaose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Apis mellifera | alpha-D-glucose + ? | - |
? | |
maltose + H2O | - |
Apis mellifera | alpha-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Apis mellifera | alpha-D-glucose | - |
? | |
maltotriose + H2O | best substrate | Apis mellifera | maltose + alpha-D-glucose | - |
? | |
additional information | maltooligosachharides of four or more units of polymerization are slowly hydrolyzed | Apis mellifera | ? | - |
? | |
nigerose + H2O | - |
Apis mellifera | 2 alpha-D-glucose | - |
? | |
phenyl alpha-D-glucoside + H2O | - |
Apis mellifera | phenol + alpha-D-glucose | - |
? | |
phenyl maltoside + H2O | - |
Apis mellifera | phenol + alpha-D-glucose | - |
? | |
sucrose + H2O | - |
Apis mellifera | alpha-D-glucose + beta-D-fructose | - |
? | |
turanose + H2O | - |
Apis mellifera | alpha-D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Apis mellifera |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase III | - |
Apis mellifera |
HBG III | - |
Apis mellifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Apis mellifera |
60 | - |
15 min, complete inactivation | Apis mellifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Apis mellifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Apis mellifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
labile below pH 4.5 | Apis mellifera |
5 | 10 | stable | Apis mellifera |