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Literature summary for 3.2.1.20 extracted from

  • Tanaka, Y.; Aki, T.; Hidaka, Y.; Furuya, Y.; Kawamoto, S.; Shigeta, S.; Ono, K.; Suzuki, O.
    Purification and characterization of a novel fungal alpha-glucosidase from Mortierella alliacea with high starch-hydrolytic activity (2002), Biosci. Biotechnol. Biochem., 66, 2415-2423.
    View publication on PubMed
Search for more literature for 3.2.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Mortierella alliacea
2.4
-
 maltotetraose pH 5.0, 37°C Mortierella alliacea
2.7
-
 maltotriose pH 5.0, 37°C Mortierella alliacea
2.8
-
 maltopentaose pH 5.0, 37°C Mortierella alliacea
2.9
-
 maltohexaose pH 5.0, 37°C Mortierella alliacea
3.5
-
 maltose pH 5.0, 37°C Mortierella alliacea
4.9
-
 maltoheptaose pH 5.0, 37°C Mortierella alliacea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Mortierella alliacea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
61000
-
 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
92000
-
 gel filtration Mortierella alliacea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mortierella alliacea the organism does not possess alpha-amylase activity ?
-
 ?
additional information Mortierella alliacea YN-15 the organism does not possess alpha-amylase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alliacea
-
-
-
Mortierella alliacea YN-15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant, 33fold by ethanol precipitation and 4 chromatographic steps Mortierella alliacea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
 purified enzyme, substrate isomaltose Mortierella alliacea
3.4
-
 purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Mortierella alliacea
5.3
-
 purified enzyme, substrate maltitol Mortierella alliacea
7
-
 purified enzyme, substrate kojibiose Mortierella alliacea
8.3
-
 purified enzyme, substrate amylose Mortierella alliacea
26.1
-
 purified enzyme, substrate nigerose Mortierella alliacea
29.8
-
 purified enzyme, substrate maltose Mortierella alliacea
30.2
-
 purified enzyme, substrate glycogen Mortierella alliacea
34.3
-
 purified enzyme, substrate amylopectin Mortierella alliacea
42.9
-
 purified enzyme, substrate soluble starch Mortierella alliacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Mortierella alliacea 4-nitrophenol + alpha-D-glucopyranose
-
 ?
amylopectin + H2O high activity Mortierella alliacea ?
-
 ?
amylose + H2O n = 18 Mortierella alliacea alpha-D-glucose
-
 ?
D-glucose + ethanol transglucosylation activity in presence of ethanol with several substrates, e.g. maltose, kojibiose, nigerose, glycogen, starch, overview, the enzyme favors the forward reaction Mortierella alliacea ethyl-alpha-D-glucoside
-
 r
glycogen + H2O high activity Mortierella alliacea alpha-D-glucose
-
 ?
isomaltose + H2O low activity Mortierella alliacea 2 D-glucose
-
 ?
kojibiose + H2O
-
 Mortierella alliacea alpha-D-glucose + D-glucose
-
 ?
kojibiose + H2O
-
 Mortierella alliacea YN-15 alpha-D-glucose + D-glucose
-
 ?
maltitol + H2O
-
 Mortierella alliacea ?
-
 ?
maltoheptaose + H2O
-
 Mortierella alliacea maltohexaose + D-glucose
-
 ?
maltohexaose + H2O
-
 Mortierella alliacea maltopentaose + D-glucose
-
 ?
maltopentaose + H2O
-
 Mortierella alliacea D-glucose + ?
-
 ?
maltose + H2O maltose is a glucosyl donor for transglycosylation activity, high activity Mortierella alliacea 2 D-glucose
-
 ?
maltose + H2O maltose is a glucosyl donor for transglycosylation activity, high activity Mortierella alliacea YN-15 2 D-glucose
-
 ?
maltotetraose + H2O
-
 Mortierella alliacea maltotriose + D-glucose
-
 ?
maltotriose + H2O
-
 Mortierella alliacea maltose + D-glucose
-
 ?
additional information the organism does not possess alpha-amylase activity Mortierella alliacea ?
-
 ?
additional information dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates Mortierella alliacea ?
-
 ?
additional information the organism does not possess alpha-amylase activity Mortierella alliacea YN-15 ?
-
 ?
additional information dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates Mortierella alliacea YN-15 ?
-
 ?
nigerose + H2O high activity Mortierella alliacea 2 alpha-D-glucose
-
 ?
nigerose + H2O high activity Mortierella alliacea YN-15 2 alpha-D-glucose
-
 ?
starch + H2O soluble starch, high activity, best substrate Mortierella alliacea alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
More the subunits are stably aggregated to each other even at high salt concentrations of 0.5 M Mortierella alliacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 formation of ethyl-alpha-D-glucose Mortierella alliacea
55
-
 substrate maltose Mortierella alliacea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mortierella alliacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 formation of ethyl-alpha-D-glucose Mortierella alliacea
5 6 substrate maltose Mortierella alliacea