Literature summary for 3.2.1.20 extracted from

Raasch, C.; Streit, W.; Schanzer, J.; Bibel, M.; Gosslar, U.; Liebl, W.
Thermotoga maritima AglA, an extremely thermostable NAD+-, Mn2+-, and thiol-dependent alpha-glucosidase (2000), Extremophiles, 4, 189-200.

Search for more literature for 3.2.1.20

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	slight activation, 600 mM are required for maximal activity, cannot substitute for DTT	Thermotoga maritima
DTT	dependent on reducing agents, 50 mM or more required for maximal activity, due to existence of essential sulfhydryl groups in the enzyme	Thermotoga maritima
L-cysteine	can only partially substitute for DTT with 3fold less activation at 60 mM	Thermotoga maritima
additional information	no activation by NADP+, NADH, NADPH, biotin, folic acid, riboflavin, FMN, FAD, ATP, and thiamine diphosphate	Thermotoga maritima
NAD+	dependent on, 0.9 mM required	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, genomic organization of the gene cluster, functional overexpression in Escherichia coli JM83	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Thermotoga maritima
EDTA	complete inhibition at 2 mM	Thermotoga maritima
Hg2+	98% inhibition at 0.1 mM, due to existence of essential sulfhydryl groups in the enzyme	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	4-nitrophenyl alpha-D-glucopyranoside	pH 7.0, 60°C	Thermotoga maritima
0.53	-	4-nitrophenyl alpha-D-galactopyranoside	pH 7.0, 60°C	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates, can partially substitute for Mn2+	Thermotoga maritima
Mn2+	activates, dependent on, 1 mM required	Thermotoga maritima
additional information	Fe2+ interferes with the assay, not affected by Mg2+, Ca2+, Sr2+, Ba2+, Zn2+	Thermotoga maritima
Ni2+	activates, can partially substitute for Mn2+	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	2 * 58000, SDS-PAGE	Thermotoga maritima
110000	-	gel filtration	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	O33830	enzyme AglA, gene aglA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli, by heat denaturation and ion exchange chromatography, large scale, to near homogeneity	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-galactopyranoside + H2O	-	Thermotoga maritima	4-methylumbelliferone + alpha-D-galactopyranose	-	?
4-methylumbelliferyl alpha-D-glucopyranoside + H2O	-	Thermotoga maritima	4-methylumbelliferone + alpha-D-glucopyranose	-	?
4-nitrophenyl alpha-D-galactopyranoside + H2O	-	Thermotoga maritima	4-nitrophenol + alpha-D-galactopyranose	-	?
4-nitrophenyl alpha-D-glucopyranoside + H2O	-	Thermotoga maritima	4-nitrophenol + alpha-D-glucopyranose	-	?
additional information	substrate specificity, bulky substitutents on the aglycon side are no hindrance for hydrolysis of the glycosidic bond, no activity with cyclic maltooligosaccharides, such as alpha-, beta-, or gamma-cyclodextrin	Thermotoga maritima	?	-	?
n-dodecyl-beta-D-maltoside + H2O	-	Thermotoga maritima	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 58000, SDS-PAGE	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
AglA	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
65	100	50% of maximal activity at 65°C and 100°C	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	enzyme is extremely thermostable	Thermotoga maritima
50	-	loss of 50% activity in 48 h	Thermotoga maritima
70	-	loss of 80% activity in 48 h	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.12	-	4-nitrophenyl alpha-D-glucopyranoside	pH 7.0, 60°C	Thermotoga maritima
10.48	-	4-nitrophenyl alpha-D-galactopyranoside	pH 7.0, 60°C	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.5	50% of maximal activity at pH 6.5 and pH 8.5	Thermotoga maritima

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Release 2023.1 (January 2023)