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Literature summary for 3.2.1.20 extracted from

  • Bravo-Torres, J.C.; Villagomez-Castro, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.
    Purification and biochemical characterisation of a membrane-bound alpha-glucosidase from the parasite Entamoeba histolytica (2004), Int. J. Parasitol., 34, 455-462.
    View publication on PubMed
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycine strong inhibition of hydrolysis of kojibiose, nigerose Entamoeba histolytica
3-deoxy-D-glucose strong inhibition Entamoeba histolytica
6-deoxy-D-glucose strong inhibition Entamoeba histolytica
castanospermine strong inhibition of hydrolysis of kojibiose, nigerose Entamoeba histolytica
D-galactose slight inhibition Entamoeba histolytica
D-glucose strong inhibition Entamoeba histolytica
diethyldicarbonate strong inhibition Entamoeba histolytica
additional information no inhibition by australine, mannose, and 2-deoxy-D-glucose, not affected by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide Entamoeba histolytica
NEM slight inhibition Entamoeba histolytica
phosphatidylcholine
-
 Entamoeba histolytica
phosphatidylglycerol
-
 Entamoeba histolytica
Tris buffer inactivation Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica HM1:IMSS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by solubilzation and ion exchange chromatography, 3.8fold, further purification via concanavalin A affinity Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0027
-
 partially purified enzyme Entamoeba histolytica

Storage Stability

Storage Stability Organism
4°C, partially purified enzyme, 69% remaining activity after 10 days, 30% remaining activity after 20 days Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
 Entamoeba histolytica 4-methylumbelliferol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O hydrolysis of the alpha-1,6-glycosidic bond Entamoeba histolytica 2 alpha-D-glucose
-
 ?
kojibiose + H2O hydrolysis of the alpha-1,2-glycosidic bond Entamoeba histolytica alpha-D-glucose + D-glucose
-
 ?
kojibiose + H2O hydrolysis of the alpha-1,2-glycosidic bond Entamoeba histolytica HM1:IMSS alpha-D-glucose + D-glucose
-
 ?
maltose + H2O hydrolysis of the alpha-1,4-glycosidic bond Entamoeba histolytica 2 D-glucose
-
 ?
maltose + H2O hydrolysis of the alpha-1,4-glycosidic bond Entamoeba histolytica HM1:IMSS 2 D-glucose
-
 ?
additional information no activity with cellobiose Entamoeba histolytica ?
-
 ?
additional information no activity with cellobiose Entamoeba histolytica HM1:IMSS ?
-
 ?
nigerose + H2O best substrate, hydrolysis of the alpha-1,3-glycosidic bond Entamoeba histolytica 2 alpha-D-glucose
-
 ?
nigerose + H2O best substrate, hydrolysis of the alpha-1,3-glycosidic bond Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
 ?
trehalose + H2O hydrolysis of the alpha-1,1-glycosidic bond Entamoeba histolytica ?
-
 ?
trehalose + H2O hydrolysis of the alpha-1,1-glycosidic bond Entamoeba histolytica HM1:IMSS ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Entamoeba histolytica