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Literature summary for 3.2.1.20 extracted from

  • Martino, A.; Schiraldi, C.; Fusco, S.; Di Lernia, I.; Costabile, T.; Pellicano, T.; Marotta, M.; Generoso, M.; van der Oost, J.; Sensen, C.W.; Charlebois, R.L.; Moracci, M.; Rossi, M.; De Rosa, M.
    Properties of the recombinant alpha-glucosidase from Sulfolobus solfataricus in relation to starch processing (2001), J. Mol. Catal. B, 11, 787-794.
No PubMed abstract available
Search for more literature for 3.2.1.20

Application

Application Comment Organism
nutrition usage in industrial processes to hydrolyze starch, saccharification step Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
gene SsGA, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3), cytosolic recombinant enzyme Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by selective and progressive thermal precipitation, 14.5fold to homogeneity Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.66
-
 purified recombinant enzyme, substrate maltohexaose Saccharolobus solfataricus
6.95
-
 purified recombinant enzyme, substrate maltotriose Saccharolobus solfataricus
18.8
-
 purified recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Saccharolobus solfataricus
35.6
-
 purified recombinant enzyme, substrate maltose Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 4-nitrophenol + alpha-D-glucopyranose
-
 ?
maltohexaose + H2O
-
 Saccharolobus solfataricus maltopentaose + D-glucose
-
 ?
maltohexaose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 maltopentaose + D-glucose
-
 ?
maltose + H2O
-
 Saccharolobus solfataricus 2 D-glucose
-
 ?
maltose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 2 D-glucose
-
 ?
maltotriose + H2O
-
 Saccharolobus solfataricus maltose + D-glucose
-
 ?
maltotriose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 maltose + D-glucose
-
 ?
additional information no activity with starch and amylose Saccharolobus solfataricus ?
-
 ?
additional information no activity with starch and amylose Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 50% remaining activity after 40-70 h, half-life: 156 h Saccharolobus solfataricus
85
-
 completely stable up to for 2 h, half-life: 150 h Saccharolobus solfataricus
90
-
 loss of 21.2% activity within 2 h Saccharolobus solfataricus
100
-
 loss of 55.2% activity within 2 h, half-life: 80 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8.5 completely stable Saccharolobus solfataricus