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Literature summary for 3.2.1.20 extracted from
- Enzyme kinetic properties of alpha-1,4-glucosidase in Bacillus thuringiensis (2004), Biochem. Eng. J., 17, 121-128.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced by malto-oligosaccharides, and more weakly by maltose, during vegetative growth | Bacillus thuringiensis serovar kurstaki |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside | substrate inhibition at concentrations above 2.1 mM | Bacillus thuringiensis serovar kurstaki |  |
| acarbose | almost complete inhibition at 0.114 mM | Bacillus thuringiensis serovar kurstaki | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus thuringiensis serovar kurstaki | |
| 0.37 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 7.0, 21-22°C | Bacillus thuringiensis serovar kurstaki | |
| 4.3 | - |
malto-oligosaccharides | pH 7.0, 30°C | Bacillus thuringiensis serovar kurstaki | |
| 14 | - |
maltose | pH 7.0, 30°C | Bacillus thuringiensis serovar kurstaki | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Bacillus thuringiensis serovar kurstaki | 5622 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malto-oligosaccharides + H2O | Bacillus thuringiensis serovar kurstaki | - |
alpha-D-glucose | - |
? | |
| malto-oligosaccharides + H2O | Bacillus thuringiensis serovar kurstaki HD-1 | - |
alpha-D-glucose | - |
? | |
| maltose + H2O | Bacillus thuringiensis serovar kurstaki | - |
alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | Bacillus thuringiensis serovar kurstaki HD-1 | - |
alpha-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis serovar kurstaki | - |
- |
- |
| Bacillus thuringiensis serovar kurstaki HD-1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus thuringiensis serovar kurstaki |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | preferred substrate | Bacillus thuringiensis serovar kurstaki | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | preferred substrate | Bacillus thuringiensis serovar kurstaki HD-1 | 4-nitrophenol + alpha-D-glucose | - |
? | |
| malto-oligosaccharides + H2O | - |
Bacillus thuringiensis serovar kurstaki | alpha-D-glucose | - |
? | |
| malto-oligosaccharides + H2O | from starch | Bacillus thuringiensis serovar kurstaki | alpha-D-glucose | - |
? | |
| malto-oligosaccharides + H2O | - |
Bacillus thuringiensis serovar kurstaki HD-1 | alpha-D-glucose | - |
? | |
| malto-oligosaccharides + H2O | from starch | Bacillus thuringiensis serovar kurstaki HD-1 | alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Bacillus thuringiensis serovar kurstaki | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | - |
Bacillus thuringiensis serovar kurstaki HD-1 | alpha-D-glucose + D-glucose | - |
? | |
| additional information | no activity with sucrose and trehalose, poor activity with isomaltose | Bacillus thuringiensis serovar kurstaki | ? | - |
? | |
| additional information | no activity with sucrose and trehalose, poor activity with isomaltose | Bacillus thuringiensis serovar kurstaki HD-1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4-glucosidase | - |
Bacillus thuringiensis serovar kurstaki |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | 22 | assay at, substrate 4-nitrophenyl alpha-D-glucopyranoside | Bacillus thuringiensis serovar kurstaki |
| 30 | - |
assay at, substrate maltose or malto-oligosaccharides | Bacillus thuringiensis serovar kurstaki |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus thuringiensis serovar kurstaki |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Bacillus thuringiensis serovar kurstaki |
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