Literature summary for 3.2.1.20 extracted from

Andreotti, G.; Giordano, A.; Tramice, A.; Mollo, E.; Trincone, A.
Hydrolyses and transglycosylations performed by purified alpha-D-glucosidase of the marine mollusc Aplysia fasciata (2006), J. Biotechnol., 122, 274-284.

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General Stability

General Stability	Organism
bovine serum albumin stabilizes the purified enzyme	Aplysia fasciata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.26	-	4-nitrophenyl alpha-D-glucopyranoside	pH 5.8, 34°C	Aplysia fasciata
2.06	-	4-nitrophenyl alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranoside	pH 5.8, 34°C	Aplysia fasciata
5.7	-	maltose	pH 5.8, 34°C	Aplysia fasciata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
69000	-	4 * 69000, SDS-PAGE	Aplysia fasciata
255000	-	gel filtration	Aplysia fasciata

Organism

Organism	UniProt	Comment	Textmining
Aplysia fasciata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 25fold by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, again ion exchange chromatography, and ultrafiltration	Aplysia fasciata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38.4	-	purified enzyme	Aplysia fasciata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O	-	Aplysia fasciata	4-nitrophenol + alpha-D-glucose	-	?
4-nitrophenyl alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranoside + H2O	alpha-1,4-glycosidic bond	Aplysia fasciata	4-nitrophenol + alpha-D-glucose	-	?
isomaltose + H2O	very low activity	Aplysia fasciata	2 alpha-D-glucose	-	?
maltose + H2O	-	Aplysia fasciata	alpha-D-glucose + D-glucose	-	?
additional information	no activity in hydrolysis with starch, panose, pullulan, amylopectin, amylose, isomaltose, and saccharose, the enzyme also performs transglycosylation reactions with different donors and a variety of acceptors, product determinations, the activity with alpha-1,4-glycosidic linkages is higher compared to alpha-1,6-glycosidic likanges, substrate specificity, overview	Aplysia fasciata	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 69000, SDS-PAGE	Aplysia fasciata

Synonyms

Synonyms	Comment	Organism
alpha-D-glucosidase	-	Aplysia fasciata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
36	44	-	Aplysia fasciata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
75.9	-	4-nitrophenyl alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranoside	pH 5.8, 34°C	Aplysia fasciata
163	-	4-nitrophenyl alpha-D-glucopyranoside	pH 5.8, 34°C	Aplysia fasciata
489	-	maltose	pH 5.8, 34°C	Aplysia fasciata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.8	-	-	Aplysia fasciata

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	-	34°C, 0.009 mg/ml bovine serum albumin, 19 h, 67% remaining activity, the half-life is about 30 h	Aplysia fasciata
5.8	-	34°C, 0.009 mg/ml bovine serum albumin, 19 h, 64% remaining activity, the half-life is about 30 h	Aplysia fasciata
6.5	-	34°C, 0.009 mg/ml bovine serum albumin, 19 h, 69% remaining activity, the half-life is about 30 h	Aplysia fasciata

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Release 2023.1 (January 2023)