Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 2 mM | Geobacillus thermodenitrificans | |
EDTA | 22% inhibition at 10 mM | Geobacillus thermodenitrificans | |
iodoacetic acid | 23% inhibition at 10 mM | Geobacillus thermodenitrificans | |
additional information | no inhibition by phytate | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme exhibits Michaelis-Menten kinetics | Geobacillus thermodenitrificans | |
17 | - |
maltose | pH 7.0, 55°C | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | Geobacillus thermodenitrificans | - |
alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | Geobacillus thermodenitrificans HRO10 | - |
alpha-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans HRO10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 25.4fold to homogeneity by ultrafiltration, chromatofocusing, and gel filtration | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Geobacillus thermodenitrificans | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Geobacillus thermodenitrificans HRO10 | alpha-D-glucose + D-glucose | - |
? | |
additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans HRO10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 75 | 30% of maximal activity at 35°C, 80% of maximal activity at 45-60°C, inactive above 75°C | Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, 30 min, 70% activity remaining | Geobacillus thermodenitrificans |
70 | - |
purified enzyme, 20 min, 3.7% activity remaining | Geobacillus thermodenitrificans |
75 | - |
inactivation | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Geobacillus thermodenitrificans |