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Literature summary for 3.2.1.20 extracted from

  • Wongchawalit, J.; Yamamoto, T.; Nakai, H.; Kim, Y.M.; Sato, N.; Nishimoto, M.; Okuyama, M.; Mori, H.; Saji, O.; Chanchao, C.; Wongsiri, S.; Surarit, R.; Svasti, J.; Chiba, S.; Kimura, A.
    Purification and characterization of alpha-glucosidase I from Japanese honeybee (Apis cerana japonica) and molecular cloning of its cDNA (2006), Biosci. Biotechnol. Biochem., 70, 2889-2898.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Apis cerana japonica
DNA and amino acid sequence determination and anaylsis Apis cerana japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information alpha-glucosidase I shows negative cooperative behaviour with substrates sucrose, maltose, and 4-nitrophenyl-alpha-D-glucopyranoside, but positive cooperativity with substrate turanose Apis cerana japonica
0.21
-
p-nitrophenyl alpha-D-glucoside
-
Apis cerana japonica
0.21
-
4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
0.4
-
2-((2-(guanin-9-yl)ethyl)(2-((2-hydroxyethyl)((2-phosphonoethyl)amino)ethyl)amino)ethyl)phosphonic acid
-
Apis cerana japonica
0.4
-
maltotriose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
1.3
-
maltotetraose
-
Apis cerana japonica
1.3
-
maltotetraose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
1.5
-
maltose
-
Apis cerana japonica
1.5
-
maltose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
2.4
-
sucrose
-
Apis cerana japonica
5.5
-
maltopentaose
-
Apis cerana japonica
5.5
-
maltopentaose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
8.7
-
kojibiose
-
Apis cerana japonica
8.7
-
kojibiose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66515
-
1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica
74000
-
1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica
82000
-
alpha-glucosidase I, native PAGE Apis cerana japonica

Organism

Organism UniProt Comment Textmining
Apis cerana japonica A1IHL0
-
-
Apis cerana japonica A1IHL0 alpha-glucosidase I; Japanese honeybee
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein alpha-glucosidase I contains 15% carbohydrate Apis cerana japonica

Purification (Commentary)

Purification (Comment) Organism
by salting-out chromatography and gel filtration Apis cerana japonica
native alpha-glucosidase I by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration Apis cerana japonica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
crude extract Apis cerana japonica
13
-
purified enzyme Apis cerana japonica
13
-
purified alpha-glucosidase I Apis cerana japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Apis cerana japonica 4-nitrophenol + alpha-D-glucose
-
?
4-nitrophenyl alpha-D-glucoside + H2O
-
Apis cerana japonica 4-nitrophenol + D-glucose
-
?
kojibiose + H2O
-
Apis cerana japonica alpha-D-glucose + D-glucose
-
?
kojibiose + H2O
-
Apis cerana japonica ?
-
?
maltopentaose + H2O
-
Apis cerana japonica ?
-
?
maltopentaose + H2O
-
Apis cerana japonica alpha-D-glucose + ?
-
?
maltose + H2O
-
Apis cerana japonica ?
-
?
maltose + H2O
-
Apis cerana japonica alpha-D-glucose + D-glucose
-
?
maltotetraose + H2O
-
Apis cerana japonica ?
-
?
maltotetraose + H2O
-
Apis cerana japonica alpha-D-glucose
-
?
maltotriose + H2O
-
Apis cerana japonica ?
-
?
maltotriose + H2O
-
Apis cerana japonica maltose + alpha-D-glucose
-
?
additional information no activity toward isomaltose, nigerose or soluble starch Apis cerana japonica ?
-
?
additional information substrate specificity, overview, the enzyme is also active with sucrose and turanose Apis cerana japonica ?
-
?
sucrose + H2O
-
Apis cerana japonica D-fructose + D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 82000, alpha-glucosidase I, SDS-PAGE Apis cerana japonica
monomer 1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica

Synonyms

Synonyms Comment Organism
alpha-glucosidase I
-
Apis cerana japonica
alpha-glucoside glucohydrolase
-
Apis cerana japonica
JHGase I
-
Apis cerana japonica
maltase
-
Apis cerana japonica
More the enzyme belongs to the alpha-amylase enzyme family Apis cerana japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Apis cerana japonica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
-
Apis cerana japonica
40
-
purified alpha-glucosidase I, 15 min, stable up to Apis cerana japonica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.7
-
kojibiose
-
Apis cerana japonica
8.7
-
kojibiose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
13
-
maltotetraose
-
Apis cerana japonica
13
-
p-nitrophenyl alpha-D-glucoside
-
Apis cerana japonica
13
-
maltotriose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
13
-
maltopentaose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
13
-
4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
15
-
maltotriose
-
Apis cerana japonica
15
-
maltopentaose
-
Apis cerana japonica
15
-
maltotetraose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
22
-
maltose
-
Apis cerana japonica
22
-
maltose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
40
-
sucrose
-
Apis cerana japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Apis cerana japonica
5
-
alpha-glucosidase I Apis cerana japonica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10.5
-
Apis cerana japonica
4.5 10.5 purified alpha-glucosidase I, 24 h, 4°C, stable within this range Apis cerana japonica