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Literature summary for 3.2.1.20 extracted from
- Enzymatic properties of alpha- and beta-glucosidases extracted from midgut and salivary glands of rice striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae) (2009), C. R. Biol., 332, 633-641.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 40 mmol/l, 225.53% relative enzyme activity in midgut, 190.24% relative enzyme activity in salivary glands | Chilo suppressalis |  |
| MgCl2 | 40 mmol/l, 206.2% relative enzyme activity in midgut, 166% relative enzyme activity in salivary glands | Chilo suppressalis | |
| NaCl | 40 mmol/l, 223.25% relative enzyme activity in midgut, 193.54% relative enzyme activity in salivary glands | Chilo suppressalis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 4 mmol/l, 63.27% relative enzyme activity in midgut, 70% relative enzyme activity in salivary glands | Chilo suppressalis | |
| KCl | 40 mmol/l, 36.04% relative enzyme activity in midgut, 29.26% relative enzyme activity in salivary glands | Chilo suppressalis | |
| SDS | 8 mmol/l, 86.72% relative enzyme activity in midgut, 60.41% relative enzyme activity in salivary glands | Chilo suppressalis | |
| Urea | 6000 mmol/l, 26.74% relative enzyme activity in midgut, 38.26% relative enzyme activity in salivary glands | Chilo suppressalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.24 | - |
p-nitrophenyl-alpha-D-glucopyranoside | salivary gland | Chilo suppressalis | |
| 1.71 | - |
p-nitrophenyl-alpha-D-glucopyranoside | midgut | Chilo suppressalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chilo suppressalis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Chilo suppressalis | - |
| midgut | - |
Chilo suppressalis | - |
| salivary gland | - |
Chilo suppressalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000063 | - |
in salivary gland | Chilo suppressalis |
| 0.000009 | - |
in midgut | Chilo suppressalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | assay at pH 5.0, 37°C, reaction stopped by adding sodium carbonate | Chilo suppressalis | 4-nitrophenol + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucosidase | - |
Chilo suppressalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Chilo suppressalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Chilo suppressalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH | Chilo suppressalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH | Chilo suppressalis |
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