Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.20 extracted from

  • Jones, K.; Sim, L.; Mohan, S.; Kumarasamy, J.; Liu, H.; Avery, S.; Naim, H.Y.; Quezada-Calvillo, R.; Nichols, B.L.; Pinto, B.M.; Rose, D.R.
    Mapping the intestinal alpha-glucogenic enzyme specificities of starch digesting maltase-glucoamylase and sucrase-isomaltase (2011), Bioorg. Med. Chem., 19, 3929-3934.
    View publication on PubMed
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
N- and C-terminal catalytic subunits, expression of His6-tagged N-terminal subunit ntMGAM in Drosophila S2 cells, expression of His6-tagged C-terminal subunits ctMGAMN2 and ctMGAMN20 in Spodoptera frugiperda Sf9 cells Homo sapiens
recombinant expression of N-terminally His-tagged N-terminal domain in Drosophila melanogaster S2 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
 Homo sapiens
blintol selenium analogue of salacinol Homo sapiens
de-O-sulfonated kotalanol isolated from Salacia reticulata Homo sapiens
kotalanol isolated from Salacia reticulata Homo sapiens
maltotriose exhibits strong substrate inhibition; strong substrate inhibition Homo sapiens
miglitol
-
 Homo sapiens
additional information inhibition profiles of the individual N- and C-terminal catalytic subunits of the enzyme by clinical glucosidase inhibitors, acarbose and miglitol, and glucosidase inhibitors from an Ayurvedic remedy used for the treatment of type II diabetes, overview; inhibition profiles of the individual N- and C-terminal catalytic subunits of the enzyme, overview Homo sapiens
salacinol isolated from Salacia reticulata Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.59
-
 maltose catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
1.91
-
 maltose catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
4.3
-
 maltose catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose + H2O Homo sapiens
-
 2 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged C-terminal subunits ctMGAMN2 and ctMGAMN20 from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, recombinant His6-tagged N-terminal subunit ntMGAM from Drosophila S2 by cobalt affinity chromatography and ion exchange chromatography Homo sapiens
recombinant N-terminally His-tagged N-terminal domain from Drosophila melanogaster S2 cells by copper affinity chromatography and ion exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Homo sapiens
-
small intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Homo sapiens 2 D-glucose
-
 ?
additional information the enzyme is active with maltose, maltotriose, and sucrose Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
maltase-glucoamylase
-
 Homo sapiens
MGAM
-
 Homo sapiens
More the enzyme is a family 31 glycoside hydrolase Homo sapiens
More the enzyme belongs to the family 31 of glycoside hydrolases Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000009
-
 acarbose C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000009
-
 acarbose catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.000013
-
 blintol C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.000013
-
 blintol catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.000018
-
 blintol C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000018
-
 blintol catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.000026
-
 de-O-sulfonated kotalanol C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000026
-
 de-O-sulfonated kotalanol catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.000028
-
 acarbose C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.000028
-
 acarbose catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.00003
-
 de-O-sulfonated kotalanol catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.00003
-
 de-O-sulfonated kotalanol N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens
0.000058
-
 salacinol C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.000058
-
 salacinol catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.000064
-
 kotalanol C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000064
-
 kotalanol catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.000078
-
 de-O-sulfonated kotalanol C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.000078
-
 de-O-sulfonated kotalanol catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.000092
-
 kotalanol C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.000092
-
 kotalanol catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.00019
-
 salacinol catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.00019
-
 kotalanol catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.00019
-
 salacinol N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens
0.00019
-
 kotalanol N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens
0.000211
-
 miglitol C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000211
-
 miglitol catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.000213
-
 salacinol C-terminal catalytic domain N2, pH and temperature not specified in the publication Homo sapiens
0.000213
-
 salacinol catalytic subunit ctMGAMN2, pH and temperature not specified in the publication Homo sapiens
0.00023
-
 miglitol C-terminal catalytic domain N20, pH and temperature not specified in the publication Homo sapiens
0.00023
-
 miglitol catalytic subunit ctMGAMN20, pH and temperature not specified in the publication Homo sapiens
0.00049
-
 blintol catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.00049
-
 blintol N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens
0.001
-
 miglitol catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.001
-
 miglitol N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens
0.062
-
 acarbose catalytic subunit ntMGAM, pH and temperature not specified in the publication Homo sapiens
0.062
-
 acarbose N-terminal catalytic domain, pH and temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the final step of starch hydrolysis Homo sapiens