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Literature summary for 3.2.1.20 extracted from
- Isolation and characterization of glucoamylase from a wastewater treatment yeast Hansenula fabianii J640, and construction of expression vector (2011), Appl. Microbiol. Biotechnol., 90, 981-987.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| by screening of the cDNA library and the genomic DNA library, DNA and amino acid sequence determination and analysis | Cyberlindnera fabianii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Cyberlindnera fabianii |  |
| Cu2+ | - |
Cyberlindnera fabianii | |
| Hg2+ | - |
Cyberlindnera fabianii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cyberlindnera fabianii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Cyberlindnera fabianii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Cyberlindnera fabianii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera fabianii | E1CJT1 | - |
- |
| Cyberlindnera fabianii J640 | E1CJT1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme by ultrafiltration and anion exchange chromatography | Cyberlindnera fabianii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | - |
Cyberlindnera fabianii | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | - |
Cyberlindnera fabianii J640 | alpha-D-glucose + D-glucose | - |
? | |
| additional information | the enzyme shows no ability to bind to or digest raw starch | Cyberlindnera fabianii | ? | - |
? | |
| additional information | the enzyme shows no ability to bind to or digest raw starch | Cyberlindnera fabianii J640 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Cyberlindnera fabianii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Cyberlindnera fabianii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cyberlindnera fabianii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Cyberlindnera fabianii |
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Release 2023.1 (January 2023)
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