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Literature summary for 3.2.1.20 extracted from
- Effects of mutation of Asn694 in Aspergillus niger alpha-glucosidase on hydrolysis and transglucosylation (2017), Appl. Microbiol. Biotechnol., 101, 6399-6408 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Agl, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Pichia pastoris | Aspergillus niger |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N694A | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type | Aspergillus niger |
| N694F | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased compared to the wild-type enzyme | Aspergillus niger |
| N694L | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased and the thermal stability slightly reduced compared to the wild-type enzyme | Aspergillus niger |
| N694W | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics for transglucosylation of wild-type and mutant enzymes, overview | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | A0PCH8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Pichia pastoris by nickel affinity chromatography | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120 | - |
enzyme mutant N694L, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
| 152 | - |
enzyme mutant N694A, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
| 155 | - |
enzyme mutant N694W, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
| 185 | - |
enzyme mutant N694F, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | - |
Aspergillus niger | 2 D-glucose | - |
? |  |
| additional information | transglucosylation activity by wild-type and Asn694 mutant enzymes forming isomaltooligosaccharides, overview. Isomaltose and panose are the predominant final products of wild-type alpha-glucosidase | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | alpha-glucosidases structure comparisons, overview | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGL | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
wild-type enzyme | Aspergillus niger |
| 4.7 | - |
mutant N694F | Aspergillus niger |
| 4.8 | - |
mutant N694L | Aspergillus niger |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 31, GH31 | Aspergillus niger |
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