Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not activated by D-glucose, trehalose, or delta-gluconolactone | Olea europaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | no activity is detected after 1 h of incubation at 1mM Ag+ | Olea europaea | |
Cu2+ | 21% residual activity is detected after 24 h of incubation at 1mM Cu2+ | Olea europaea | |
Hg2+ | no activity is detected after 1 h of incubation at 1mM Hg2+ | Olea europaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by K+, Mg2+, Ca2+, and Zn2+ | Olea europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65400 | - |
- |
Olea europaea |
130000 | - |
gel filtration | Olea europaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleuropein + H2O | Olea europaea | 100% activity | oleuropein aglycone + D-glucopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Olea europaea | - |
cultivar Picual | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Hitrap Q column chromatography, and HiTrap butyl Sepharose column chromatography | Olea europaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Olea europaea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
430.3 | - |
crude extract, at pH 5.5 and 45°C | Olea europaea |
3500 | - |
after 8.1fold purification, at pH 5.5 and 45°C | Olea europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | the enzyme is 200fold less active against 4-nitrophenyl-beta-D-glucopyranoside than oleuropein | Olea europaea | 4-nitrophenol + D-glucose | - |
? | |
apigenin 7-O-beta-D-glucopyranoside + H2O | 1% activity compared to oleuropein | Olea europaea | apigenin + D-glucose | - |
? | |
demethyloleuropein + H2O | 21% activity compared to oleuropein | Olea europaea | (2R,3E,4S)-4-[2-[2-(3,4-dihydroxyphenyl)ethoxy]-2-oxoethyl]-3-ethylidene-2-hydroxy-3,4-dihydro-2H-pyran-5-carboxylic acid + D-glucose | - |
? | |
ligstroside + H2O | 65.4% activity compared to oleuropein | Olea europaea | methyl (2R,3E,4S)-3-ethylidene-2-hydroxy-4-[2-[2-(4-hydroxyphenyl)ethoxy]-2-oxoethyl]-3,4-dihydro-2H-pyran-5-carboxylate + D-glucose | - |
? | |
luteolin 7-O-beta-D-glucopyranoside + H2O | 0.2% activity compared to oleuropein | Olea europaea | luteolin + D-glucose | - |
? | |
additional information | the enzyme is not active on verbascoside and rutin | Olea europaea | ? | - |
? | |
oleuropein + H2O | 100% activity | Olea europaea | oleuropein aglycone + D-glucopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 65400, SDS-PAGE | Olea europaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Olea europaea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 60% activity between 30 and 50°C | Olea europaea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable for about 1 h at up to 40°C with a dramatic decrease of activity above this temperature | Olea europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Olea europaea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | about 60% activity at pH 4.0, about 80% activity at pH 5.0, 100% activity at pH 5.5, about 40% activity at pH 6.0 | Olea europaea |