Literature summary for 3.2.1.207 extracted from

Kaewmuangmoon, J.; Kilaso, M.; Leartsakulpanich, U.; Kimura, K.; Kimura, A.; Chanchao, C.
Expression of a secretory beta-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization (2013), BMC Biotechnol., 13, 16 .

Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Apis cerana indica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
15.3	-	sucrose	pH 5.5, 37°C	Apis cerana indica
26.7	-	maltotriose	pH 5.5, 37°C	Apis cerana indica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Apis cerana indica	-	-

Organism

Organism	UniProt	Comment	Textmining
Apis cerana indica	J9SJ08	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
egg	and larva, lower transcript expression level than in larvae and pupae	Apis cerana indica	-
larva	and pupa, highest transcript expression	Apis cerana indica	-
pupa	and larva, highest transcript expression	Apis cerana indica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltotriose + 2 H2O	-	Apis cerana indica	3 D-glucose	-	?
sucrose + H2O	-	Apis cerana indica	2 D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
HGBase II	-	Apis cerana indica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Apis cerana indica

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	55	more than 80% of maximum activity	Apis cerana indica

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	65	more than 80% of maximum activity	Apis cerana indica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
26.6	-	maltotriose	pH 5.5, 37°C	Apis cerana indica
845	-	sucrose	pH 5.5, 37°C	Apis cerana indica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	-	-	Apis cerana indica

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.5	5	more than 80% of maximum activity	Apis cerana indica

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	8	more than 80% of maximum activity	Apis cerana indica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1	-	maltotriose	pH 5.5, 37°C	Apis cerana indica
55.4	-	sucrose	pH 5.5, 37°C	Apis cerana indica

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Release 2023.1 (January 2023)