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Literature summary for 3.2.1.21 extracted from
- Purification and properties of three cellobiases from Aspergillus niger A20 (1999), Appl. Biochem. Biotechnol., 76, 33-44.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | slight inhibition at 5-10 mM | Aspergillus niger |  |
| Cd2+ | slight inhibition at 5-10 mM | Aspergillus niger | |
| Cu2+ | slight inhibition at 5-10 mM | Aspergillus niger | |
| Hg2+ | complete inhibition at 5 mM | Aspergillus niger | |
| Iodine | complete inactivation at 5 mM | Aspergillus niger | |
| Zn2+ | slight inhibition at 5-10 mM | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
cellobiose | for cellobiase A | Aspergillus niger | |
| 1 | - |
cellobiose | for cellobiase C | Aspergillus niger | |
| 1.36 | - |
cellobiose | cellobiase B | Aspergillus niger | |
| 1.4 | - |
2-Nitrophenyl-beta-D-glucopyranoside | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulation of cellobiases B and C at 5-10 mM | Aspergillus niger | |
| Co2+ | stimulation for cellobiase A at 5-10 mM | Aspergillus niger | |
| Mg2+ | stimulation of cellobiase A at 5-10 mM | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
cellobiase C, SDS-PAGE | Aspergillus niger |
| 80000 | - |
cellobiase B, SDS-PAGE | Aspergillus niger |
| 88000 | - |
cellobiase A, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Aspergillus niger | low activity | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Aspergillus niger | lactose | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus niger | cellobiose | beta-D-glucose + D-glucose | additional product: cellotetraose as the product of transglycosylation | r | |
| dextran + H2O | Aspergillus niger | very low activity | beta-D-glucose + ? | - |
? | |
| starch + H2O | Aspergillus niger | low activity | beta-D-glucose + ? | - |
? | |
| xylan + H2O | Aspergillus niger | very low activity | beta-D-glucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content, w/w: 8.8 cellobiase A, 9.4 cellobiase B, 7.2 cellobiase C | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | 3 isoenzmes, A, B and C | Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 119.3 | - |
cellobiase B | Aspergillus niger |
| 140.4 | - |
cellobiase C | Aspergillus niger |
| 184.1 | - |
cellobiase A | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-galactoside + H2O | low activity | Aspergillus niger | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Aspergillus niger | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Aspergillus niger | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | low activity | Aspergillus niger | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Aspergillus niger | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus niger | beta-D-glucose + D-glucose | additional product: cellotetraose as the product of transglycosylation | r | |
| carboxymethyl-cellulose + H2O | low activity | Aspergillus niger | beta-D-glucose + ? | - |
? | |
| dextran + H2O | very low activity | Aspergillus niger | beta-D-glucose + ? | - |
? | |
| starch + H2O | low activity | Aspergillus niger | beta-D-glucose + ? | - |
? | |
| xylan + H2O | very low activity | Aspergillus niger | beta-D-glucose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
cellobiases A, B | Aspergillus niger |
| 60 | - |
cellobiase C | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life of cellobiase A: 27 min, cellobiase B: 32.3 min | Aspergillus niger |
| 60 | - |
half-life for cellobiase C: 45 min | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Aspergillus niger |
| 4.5 | - |
cellobiases A, B, C | Aspergillus niger |
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