Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | 50% increase of activity | Thermomyces lanuginosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | competitive inhibition, Ki: 0.55 mM | Thermomyces lanuginosus | |
cellobiose | competitive inhibition, Ki: 0.52 mM | Thermomyces lanuginosus | |
Hg2+ | complete inhibition at 2 mM | Thermomyces lanuginosus | |
N-bromosuccinimide | complete inhibition at 2 mM | Thermomyces lanuginosus | |
Salicin | competitive inhibition, Ki: 0.81 mM | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
2 * 101000, SDS-PAGE | Thermomyces lanuginosus |
200000 | - |
gel filtration | Thermomyces lanuginosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-alpha-D-glucopyranosyl-D-glucose | Thermomyces lanuginosus | maltose | alpha-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | gentiobiose | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Thermomyces lanuginosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Thermomyces lanuginosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.65 | - |
- |
Thermomyces lanuginosus |
Storage Stability | Organism |
---|---|
4°C, 20 mM Tris-HCl, pH 7.1, 4 months | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Thermomyces lanuginosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Thermomyces lanuginosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Thermomyces lanuginosus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Thermomyces lanuginosus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl-alpha-D-glucoside | less, but significant hydrolysis | Thermomyces lanuginosus | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-O-alpha-D-glucopyranosyl-D-glucose | maltose | Thermomyces lanuginosus | alpha-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 101000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable within 30 min | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
rapid decrease of activity above | Thermomyces lanuginosus |