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Literature summary for 3.2.1.21 extracted from

  • Ohnishi, M.; Okada, G.; Yazaki, T.
    Characterization of the subsite structure of the beta-glucosidase from Aspergillus niger, an aspect of the mechanism of carbohydrate recognition (1998), Carbohydr. Res., 308, 201-205.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
4-nitrophenyl-beta-D-glucopyranoside hydrophobicity of the aglycon in the 4-position takes a substantial role in the binding of the substrate at subsite 2 of the 2 main binding-modes Aspergillus niger
0.85
-
cellobiose
-
Aspergillus niger
2.1
-
4-hydroxyphenyl-beta-D-glucopyranoside hydrophobicity of the aglycon takes a substantial role in the binding of the substrate at subsite 2 of the 2 main binding-modes Aspergillus niger
3.5
-
benzyl-beta-D-glucopyranoside hydrophobicity of the aglycon takes a substantial role in the binding of the substrate at subsite 2 of the 2 main binding-modes Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger hydrophobic-driven binding of the glucose residue at subsite 2 of 2 main binding-modes of beta-glucosidase beta-D-glucose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Aspergillus niger 4-hydroxyphenol + beta-D-glucopyranose
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 2-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 4-nitrophenol + D-glucopyranose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus niger beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O hydrophobic-driven binding of the glucose residue at subsite 2 of 2 main binding-modes of beta-glucosidase Aspergillus niger beta-D-glucose + D-glucose
-
?
benzyl-beta-D-glucopyranoside + H2O
-
Aspergillus niger benzyl alcohol + beta-D-glucopyranose
-
?