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Literature summary for 3.2.1.21 extracted from

  • Christakopoulos, P.; Goodenough, P.W.; Kekos, D.; Macris, B.J.; Claeyssens, M.; Bhat, M.K.
    Purification and characterization of an extracellular beta-glucosidase with transglycosylation and exo-glucosidase activities from Fusarium oxysporum (1994), Eur. J. Biochem., 224, 379-385.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
1-butanol 2.2fold increase of activity at 0.5 M Fusarium oxysporum
methanol 1.4fold increase of activity at 1 M Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 2.05 mM Fusarium oxysporum
D-glucono-1,5-lactone competitive inhibition, Ki: 0.003 mM Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Fusarium oxysporum
1.8
-
 cellobiose
-
 Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Fusarium oxysporum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Fusarium oxysporum laminaribiose beta-D-glucose + D-glucose
-
 r
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Fusarium oxysporum transglycoslation activity beta-D-glucose + D-glucose
-
 r
4-O-beta-D-galactopyranosyl-D-glucose + H2O Fusarium oxysporum lactose beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Fusarium oxysporum cellobiose beta-D-glucose + D-glucose
-
 r
4-O-beta-D-glucopyranosyl-D-glucose + H2O Fusarium oxysporum transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Fusarium oxysporum gentiobiose beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Fusarium oxysporum transglycosylation reaction beta-D-glucose + D-glucose
-
 r
cellopentaose + 4 H2O Fusarium oxysporum
-
 5 D-glucose
-
 ?
cellotetraose + 3 H2O Fusarium oxysporum
-
 4 D-glucose
-
 ?
cellotriose + 2 H2O Fusarium oxysporum
-
 3 D-glucose
-
 ?
laminarin + H2O Fusarium oxysporum
-
 beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step chromatography Fusarium oxysporum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
-
 Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Fusarium oxysporum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Fusarium oxysporum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Fusarium oxysporum 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
(R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin Fusarium oxysporum HCN + ?
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Fusarium oxysporum 2-nitrophenol + D-glucopyranose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O transglycoslation activity Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
4-methylumbelliferyl-beta-D-glucoside + H2O hydrolysis of glucose residues from both ends in an exo-fashion Fusarium oxysporum 4-methylumbelliferone + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Fusarium oxysporum 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl-alpha-D-glucoside less, but significant hydrolysis Fusarium oxysporum 4-nitrophenol + alpha-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Fusarium oxysporum beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
4-O-beta-D-glucopyranosyl-D-glucose + H2O transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O transglycosylation reaction Fusarium oxysporum beta-D-glucose + D-glucose
-
 r
cellobiose + H2O
-
 Fusarium oxysporum 2 beta-D-glucose
-
 ?
cellopentaose + 4 H2O
-
 Fusarium oxysporum 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Fusarium oxysporum 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Fusarium oxysporum 3 D-glucose
-
 ?
laminarin + H2O
-
 Fusarium oxysporum beta-D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Fusarium oxysporum