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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of two extracellular beta-glucosidases from Trichoderma reesei (1992), Biochim. Biophys. Acta, 1121, 54-60.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | competitive inhibition, Ki for beta-glucosidase I: 0.62 mM | Trichoderma reesei |  |
| Fe2+ | slight inhibition at 1 mM | Trichoderma reesei | |
| Hg2+ | slight inhibition at 1 mM only for beta-glucosidase I | Trichoderma reesei | |
| p-chloromercuribenzoic acid | strong inhibition at 1 mM | Trichoderma reesei | |
| Sn2+ | slight inhibition at 1 mM | Trichoderma reesei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase II | Trichoderma reesei | |
| 0.18 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Trichoderma reesei | |
| 2.1 | - |
cellobiose | beta-glucosidase I | Trichoderma reesei | |
| 11.1 | - |
cellobiose | beta-glucosidase II | Trichoderma reesei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
beta-glucosidase I, SDS-PAGE | Trichoderma reesei |
| 114000 | - |
beta-glucosidase II, SDS-PAGE | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Trichoderma reesei | sophorose | beta-D-glucose + D-glucose | - |
? | |
| 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Trichoderma reesei | lower activity for beta-glucosidase II | beta-D-glucose + D-glucose | - |
? | |
| 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Trichoderma reesei | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
| 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Trichoderma reesei | lower activity for beta-glucosidase II | beta-D-glucose + D-glucose | - |
? | |
| 4-O-alpha-D-glucopyranosyl-D-glucose | Trichoderma reesei | maltose | alpha-D-glucose + D-glucose | - |
? | |
| 4-O-alpha-D-glucopyranosyl-D-glucose | Trichoderma reesei | only beta-glucosidase II | alpha-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Trichoderma reesei | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Trichoderma reesei | low activity | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Trichoderma reesei | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | beta-glucosidase I, 0.12%, w/w, carbohydrate, beta-glucosidase II, 9%, w/w, carbohydrate | Trichoderma reesei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Trichoderma reesei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | 2 enzymes, beta-glucosidases I and II | Trichoderma reesei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65.3 | - |
beta-glucosidase II | Trichoderma reesei |
| 88 | - |
beta-glucosidase I | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Trichoderma reesei | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | lower activity for beta-glucosidase II | Trichoderma reesei | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Trichoderma reesei | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | lower activity for beta-glucosidase II | Trichoderma reesei | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 2-nitrophenol + D-glucopyranose | - |
? | |
| 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | lower activity for beta-glucosidase II | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | lower activity for beta-glucosidase II | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-O-alpha-D-glucopyranosyl-D-glucose | maltose | Trichoderma reesei | alpha-D-glucose + D-glucose | - |
? | |
| 4-O-alpha-D-glucopyranosyl-D-glucose | only beta-glucosidase II | Trichoderma reesei | alpha-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Trichoderma reesei | 2 beta-D-glucose | - |
? | |
| methyl-beta-D-glucoside + H2O | beta-glucosidase II | Trichoderma reesei | beta-D-glucose + methanol | - |
? | |
| phloridzin + H2O | beta-glucosidase II | Trichoderma reesei | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
beta-glucosidase II | Trichoderma reesei |
| 65 | - |
beta-glucosidase I, for cellobiose | Trichoderma reesei |
| 70 | - |
beta-glucosidase I, for 4-nitrophenyl-beta-D-glucopyranoside | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
beta-glucosidase II, stable for 1 h | Trichoderma reesei |
| 60 | - |
beta-glucosidase I, stable for 1 h | Trichoderma reesei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.6 | - |
cellobiose | beta-glucosidase II | Trichoderma reesei | |
| 51.5 | - |
cellobiose | beta-glucosidase I | Trichoderma reesei | |
| 138 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase II | Trichoderma reesei | |
| 144 | - |
4-nitrophenyl-beta-D-glucopyranoside | beta-glucosidase I | Trichoderma reesei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
beta-glucosidase II | Trichoderma reesei |
| 4.6 | - |
beta-glucosidase I | Trichoderma reesei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | 8 | beta-glucosidase II | Trichoderma reesei |
| 4 | 6 | stable within, beta-glucosidase I | Trichoderma reesei |
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