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Literature summary for 3.2.1.21 extracted from

  • Wiseman, A.
    Fungal and other beta-D-glucosidases - their properties and applications (1982), Enzyme Microb. Technol., 4, 73-78.
No PubMed abstract available

Application

Application Comment Organism
synthesis increased hydrolysis of cellobiose by immobilized enzyme preparation Prunus dulcis

General Stability

General Stability Organism
increased thermal stability by immobilization on concanavalin A-Sepharose, DEAE- or CM-Sephadex Aspergillus niger
increased thermal stability by immobilization on concanavalin A-Sepharose, DEAE- or CM-Sephadex Prunus dulcis

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose
-
Acetivibrio thermocellus
beta-D-glucose
-
Aspergillus niger
beta-D-glucose
-
Aspergillus oryzae
beta-D-glucose
-
Aspergillus wentii
beta-D-glucose
-
Homo sapiens
beta-D-glucose
-
Malus domestica
beta-D-glucose
-
Pantoea agglomerans
beta-D-glucose
-
Prunus dulcis
beta-D-glucose
-
Pyrus communis
beta-D-glucose
-
Rhizomucor miehei
beta-D-glucose
-
Saccharomyces cerevisiae
beta-D-glucose
-
Trichoderma reesei
conduritol-B-epoxide
-
Aspergillus wentii
conduritol-B-epoxide bound as an ester of (+) chiro-inositol to aspartic acid Prunus dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
4-nitrophenyl-beta-D-glucopyranoside
-
Saccharomyces cerevisiae
0.58
-
4-nitrophenyl-beta-D-glucopyranoside
-
Rhizomucor miehei
0.72
-
Salicin
-
Rhizomucor miehei
1.04
-
cellobiose
-
Rhizomucor miehei
2.1
-
4-nitrophenyl-beta-D-glucopyranoside
-
Acetivibrio thermocellus
83
-
cellobiose
-
Acetivibrio thermocellus
117
-
cellobiose increased Km if enzyme immobilized on cellulose Prunus dulcis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus niger
-
-
extracellular
-
Aspergillus oryzae
-
-
extracellular
-
Trichoderma reesei
-
-
extracellular
-
Rhizomucor miehei
-
-
extracellular
-
Aspergillus wentii
-
-
intracellular
-
Saccharomyces cerevisiae 5622
-
lysosome
-
Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
Acetivibrio thermocellus
122000
-
-
Pantoea agglomerans
218000
-
-
Aspergillus oryzae
300000
-
yeast foam strain Saccharomyces cerevisiae
313000
-
intracellular enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Saccharomyces cerevisiae cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Pantoea agglomerans cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Prunus dulcis cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Trichoderma reesei cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Rhizomucor miehei cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Malus domestica cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Pyrus communis cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Acetivibrio thermocellus cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus wentii cellobiose beta-D-glucose + D-glucose
-
?
Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae important in processing of oligosaccharides after their transfer from dolichyl diphosphate to proteins during glycoprotein synthesis Glc2Man9GlcNAc2 + beta-D-glucose
-
?
glucosyl ceramide + H2O Homo sapiens involved in normal catabolism of glucosyl ceramides sphingosine + fatty acid + beta-D-glucose
-
?
n-decyl beta-D-glucoside + H2O Saccharomyces cerevisiae 6fold higher activity than with methyl-beta-D-glucoside beta-D-glucose + n-decanol
-
?
starch + H2O Aspergillus niger
-
beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Aspergillus niger
-
-
-
Aspergillus oryzae
-
-
-
Aspergillus wentii
-
-
-
Homo sapiens
-
-
-
Malus domestica
-
-
-
Pantoea agglomerans
-
-
-
Prunus dulcis
-
var. sativa
-
Pyrus communis
-
-
-
Rhizomucor miehei
-
-
-
Saccharomyces cerevisiae
-
-
-
Trichoderma reesei
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger
glycoprotein
-
Trichoderma reesei
glycoprotein 23.4% carbohydrate content, w/w, enzymatic removal of carbohydrate moiety decreases thermal stability Rhizomucor miehei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aspergillus niger
-
cell culture
-
Aspergillus oryzae
-
cell culture
-
Rhizomucor miehei
-
cell culture
-
Acetivibrio thermocellus
-
cell culture
-
Aspergillus wentii
-
cell culture potent formation of cellobiase activity by induction by sophorose Trichoderma reesei
-
reticuloendothelial system
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Aspergillus niger 4-hydroxyphenol + beta-D-glucopyranose
-
?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Trichoderma reesei 4-hydroxyphenol + beta-D-glucopyranose
-
?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Malus domestica 4-hydroxyphenol + beta-D-glucopyranose product 4-hydroxyphenol toxic to invading pathogens ?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Pyrus communis 4-hydroxyphenol + beta-D-glucopyranose product 4-hydroxyphenol toxic to invading pathogens ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Homo sapiens 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pantoea agglomerans 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Prunus dulcis 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Trichoderma reesei 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Rhizomucor miehei 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Malus domestica 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pyrus communis 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus wentii 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O higher activity than on cellobiose Saccharomyces cerevisiae 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O less active than on cellobiose Aspergillus oryzae 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Prunus dulcis 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Aspergillus oryzae 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Aspergillus niger 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Trichoderma reesei 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Acetivibrio thermocellus 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Homo sapiens beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Saccharomyces cerevisiae beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus niger beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Pantoea agglomerans beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Prunus dulcis beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus oryzae beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Trichoderma reesei beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rhizomucor miehei beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Malus domestica beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Pyrus communis beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Acetivibrio thermocellus beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus wentii beta-D-glucose + D-glucose
-
?
cellobiose + H2O
-
Rhizomucor miehei 2 beta-D-glucose
-
?
cellobiose + H2O
-
Acetivibrio thermocellus 2 beta-D-glucose
-
?
Glc3Man9GlcNAc2 + H2O important in processing of oligosaccharides after their transfer from dolichyl diphosphate to proteins during glycoprotein synthesis Saccharomyces cerevisiae Glc2Man9GlcNAc2 + beta-D-glucose
-
?
glucosyl ceramide + H2O involved in normal catabolism of glucosyl ceramides Homo sapiens sphingosine + fatty acid + beta-D-glucose
-
?
methyl-beta-D-glucoside + H2O
-
Homo sapiens beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Saccharomyces cerevisiae beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Aspergillus niger beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Pantoea agglomerans beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Prunus dulcis beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Aspergillus oryzae beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Trichoderma reesei beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Rhizomucor miehei beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Malus domestica beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Pyrus communis beta-D-glucose + methanol
-
?
methyl-beta-D-glucoside + H2O
-
Acetivibrio thermocellus beta-D-glucose + methanol
-
?
n-decyl beta-D-glucoside + H2O 6fold higher activity than with methyl-beta-D-glucoside Saccharomyces cerevisiae beta-D-glucose + n-decanol
-
?
salicin + H2O
-
Rhizomucor miehei D-glucose + salicyl alcohol
-
?
starch + H2O
-
Aspergillus niger beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Saccharomyces cerevisiae
45
-
yeast foam strain Malus domestica
65
-
-
Trichoderma reesei
65
-
-
Rhizomucor miehei
65
-
-
Acetivibrio thermocellus
65
-
-
Aspergillus wentii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
-
Aspergillus oryzae
45
-
yeast foam strain Saccharomyces cerevisiae
50
-
-
Homo sapiens
50
-
-
Pantoea agglomerans
60
-
-
Aspergillus niger
60
-
-
Trichoderma reesei
60
-
-
Acetivibrio thermocellus
60
-
-
Aspergillus wentii
70
-
stable up to Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
Aspergillus oryzae
5.6
-
-
Prunus dulcis
6 7.5
-
Pantoea agglomerans
6
-
-
Acetivibrio thermocellus
6.4 6.8 yeast foam strain Saccharomyces cerevisiae
6.8
-
intracellular enzyme Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 10 highly active between Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7
-
Aspergillus oryzae
6 8 yeast foam strain Saccharomyces cerevisiae
6 7.5
-
Pantoea agglomerans