Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 100 mM, 69% inhibition; 100 mM, 90% inhibition | Thermoascus aurantiacus | |
CoCl2 | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
CuSO4 | 100 mM, 48% inhibition; 100 mM, 66% inhibition | Thermoascus aurantiacus | |
FeCl3 | 100 mM, 59% inhibition | Thermoascus aurantiacus | |
HgCl2 | 100 mM, 53% inhibition; 100 mM, 64% inhibition | Thermoascus aurantiacus | |
KCl | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
MgSO4 | 100 mM, 44% inhibition | Thermoascus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
p-nitrophenyl-beta-D-glucopyranoside | 80°C, pH 4.5 | Thermoascus aurantiacus | |
1.38 | - |
p-nitrophenyl-beta-D-glucopyranoside | 75°C, pH 4.0 | Thermoascus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight stimulation | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
157000 | - |
x * 157000, SDS-PAGE | Thermoascus aurantiacus |
175000 | - |
x * 175000, SDS-PAGE | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ethanol precipitation, Sephadex G-100, Q-Sepharose | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
458.9 | - |
- |
Thermoascus aurantiacus |
771 | - |
- |
Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | p-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 175000, SDS-PAGE | Thermoascus aurantiacus |
? | x * 157000, SDS-PAGE | Thermoascus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
Gl-2 | - |
Thermoascus aurantiacus |
Gl-3 | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoascus aurantiacus |
80 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | approx. 25% of maximal activity at 50°C, approx. 40% of maximal activity at 90°C | Thermoascus aurantiacus |
50 | 90 | approx. 30% of maximal activity at 50°C, approx. 20% of maximal activity at 90°C | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
25% loss of activity after 1 h | Thermoascus aurantiacus |
70 | - |
18% loss of activity after 1 h | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Thermoascus aurantiacus |
4.5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | approx. 65% of maximal activity at pH 3.0, approx. 10% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
3 | 7 | approx. 80% of maximal activity at pH 3.0, approx. 18% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | no loss of activity | Thermoascus aurantiacus |
5.5 | 8.5 | no loss of activity | Thermoascus aurantiacus |