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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a major secretory cellobiase, Cba2, from Cellulomonas biazotea (2001), Protein Expr. Purif., 23, 159-166.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
p-nitrophenyl-beta-D-glucopyranoside | 37°C, pH 7.0 | Cellulomonas biazotea |  |
| 0.73 | - |
cellobiose | 37°C, pH 7.0 | Cellulomonas biazotea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cellulomonas biazotea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 109000 | - |
x * 109000, SDS-PAGE | Cellulomonas biazotea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | Cellulomonas biazotea | - |
2 beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas biazotea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, gel filtration, anion-exchange, reversed-phase HPLC | Cellulomonas biazotea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 133 | - |
- |
Cellulomonas biazotea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | - |
Cellulomonas biazotea | 2 beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Cellulomonas biazotea | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 109000, SDS-PAGE | Cellulomonas biazotea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiase | - |
Cellulomonas biazotea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Cellulomonas biazotea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | approx. 50% of maximal activity at 30°C, approx. 15% of maximal activity at 90°C | Cellulomonas biazotea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Cellulomonas biazotea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | 7.8 | approx. 50% of maximal activity at pH 3.5, approx. 60% of maximal activity at pH 7.8 | Cellulomonas biazotea |
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