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Literature summary for 3.2.1.21 extracted from

  • Kim, S.Y.; Kim, Y.W.; Hegerl, R.; Cyrklaff, M.; Kim, I.S.
    Novel type of enzyme multimerization enhances substrate affinity of oat beta-glucosidase (2005), J. Struct. Biol., 150, 1-10.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
glucono(1-5)lactone
-
 Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of multimers of different size, overview, effects of active site modifcation by N-(dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride on kinetics, overview Avena sativa
0.063
-
 avenacoside pH 6.8, 30°C, hexameric enzyme form Avena sativa
2.3
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.8, 30°C, hexameric enzyme form Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Avena sativa 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
avenacoside + H2O Avena sativa the enzyme hydrolyzes the avenacosides to combat fungal attack C26-desgluco-avenacosides + beta-D-glucose
-
 ?
genistin + H2O Avena sativa
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 type I enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Avena sativa 4-nitrophenol + beta-D-glucose
-
 ?
avenacoside + H2O
-
 Avena sativa C26-desgluco-avenacosides + beta-D-glucose
-
 ?
avenacoside + H2O the enzyme hydrolyzes the avenacosides to combat fungal attack Avena sativa C26-desgluco-avenacosides + beta-D-glucose
-
 ?
genistin + H2O
-
 Avena sativa ?
-
 ?

Subunits

Subunits Comment Organism
More quarternary structure analysis, structure and kinetics of multimers of different size, overview Avena sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Avena sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.8, 30°C, hexameric enzyme form Avena sativa
29
-
 avenacoside pH 6.8, 30°C, hexameric enzyme form Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Avena sativa