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Literature summary for 3.2.1.21 extracted from

  • Sakai, M.; Tomita, S.; Hirata, H.; Asai, T.; Dohra, H.; Hara, M.; Watanabe, N.
    Purification and characterization of beta-glucosidase involved in the emission of 2-phenylethanol from rose flowers (2008), Biosci. Biotechnol. Biochem., 72, 219-221.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
2-phenyl-N-glucosyl-acetamidium bromide
-
 Rosa x damascena

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phenylethyl beta-D-glucopyranoside + H2O Rosa x damascena
-
 2-phenylethanol + beta-D-glucose 2-phenylethanol is a flower sent compound ?

Organism

Organism UniProt Comment Textmining
Rosa x damascena
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 36fold from petals to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and ultrafiltration Rosa x damascena

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Rosa x damascena
-
petal
-
 Rosa x damascena
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 purified enzyme Rosa x damascena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-citronellyl beta-D-glucopyranoside + H2O high activity, no activity with (S)-citronellyl beta-D-galactopyranoside or (S)-citronellyl beta-D-xylopyranoside Rosa x damascena (S)-citronellol + beta-D-glucose
-
 ?
(Z)-3-hexenyl beta-D-glucopyranoside + H2O high activity, no activity with (Z)-3-hexenyl beta-D-galactopyranoside or Z-3-hexenyl beta-D-xylopyranoside Rosa x damascena (Z)-3-hexenol + beta-D-glucose
-
 ?
2-phenylethyl beta-D-glucopyranoside + H2O
-
 Rosa x damascena 2-phenylethanol + beta-D-glucose 2-phenylethanol is a flower sent compound ?
2-phenylethyl beta-D-glucopyranoside + H2O best substrate, poor activity with 2-phenylethyl beta-D-galactopyranoside, no activity with 2-phenylethyl beta-D-xylopyranoside Rosa x damascena 2-phenylethanol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O poor activity with 4-nitrophenyl-beta-D-galactopyranoside, no activity with 4-nitrophenyl-beta-D-xylopyranoside Rosa x damascena 4-nitrophenol + beta-D-glucose
-
 ?
additional information the enzyme is highly specificic for beta-D-glucopyranosides, no activity with alpha-D-glucopyranosides Rosa x damascena ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Rosa x damascena

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified enzyme, inactivation above Rosa x damascena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rosa x damascena

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 purified enzyme, most stable at pH 7.5, inactivation below pH 5.0 Rosa x damascena