Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phenyl-N-glucosyl-acetamidium bromide | - |
Rosa x damascena |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethyl beta-D-glucopyranoside + H2O | Rosa x damascena | - |
2-phenylethanol + beta-D-glucose | 2-phenylethanol is a flower sent compound | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rosa x damascena | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 36fold from petals to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and ultrafiltration | Rosa x damascena |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Rosa x damascena | - |
petal | - |
Rosa x damascena | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
purified enzyme | Rosa x damascena |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-citronellyl beta-D-glucopyranoside + H2O | high activity, no activity with (S)-citronellyl beta-D-galactopyranoside or (S)-citronellyl beta-D-xylopyranoside | Rosa x damascena | (S)-citronellol + beta-D-glucose | - |
? | |
(Z)-3-hexenyl beta-D-glucopyranoside + H2O | high activity, no activity with (Z)-3-hexenyl beta-D-galactopyranoside or Z-3-hexenyl beta-D-xylopyranoside | Rosa x damascena | (Z)-3-hexenol + beta-D-glucose | - |
? | |
2-phenylethyl beta-D-glucopyranoside + H2O | - |
Rosa x damascena | 2-phenylethanol + beta-D-glucose | 2-phenylethanol is a flower sent compound | ? | |
2-phenylethyl beta-D-glucopyranoside + H2O | best substrate, poor activity with 2-phenylethyl beta-D-galactopyranoside, no activity with 2-phenylethyl beta-D-xylopyranoside | Rosa x damascena | 2-phenylethanol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | poor activity with 4-nitrophenyl-beta-D-galactopyranoside, no activity with 4-nitrophenyl-beta-D-xylopyranoside | Rosa x damascena | 4-nitrophenol + beta-D-glucose | - |
? | |
additional information | the enzyme is highly specificic for beta-D-glucopyranosides, no activity with alpha-D-glucopyranosides | Rosa x damascena | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rosa x damascena |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified enzyme, inactivation above | Rosa x damascena |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rosa x damascena |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | purified enzyme, most stable at pH 7.5, inactivation below pH 5.0 | Rosa x damascena |