Protein Variants | Comment | Organism |
---|---|---|
D229N | site-directed mutagenesis, mutation of the isozyme BGL1A residue from subsite +1 to the correspondent residue of isozyme BGL1B, the mutant shows decreased catalytic efficiency compared to the wild-type BGL1A | Phanerodontia chrysosporium |
D229N/K253A | site-directed mutagenesis, mutation of the isozyme BGL1A residues from subsite +1 to the correspondent residues of isozyme BGL1B, the double mutant has a hydrolytic activity at neutral pH that is restored to that of the wild-type enzyme | Phanerodontia chrysosporium |
H231D | site-directed mutagenesis, mutation of the isozyme BGL1A residue from subsite +1 to the correspondent residue of isozyme BGL1B, the mutant shows decreased catalytic efficiency compared to the wild-type BGL1A | Phanerodontia chrysosporium |
K253A | site-directed mutagenesis, mutation of the isozyme BGL1A residue from subsite +1 to the correspondent residue of isozyme BGL1B, the mutant shows decreased catalytic efficiency compared to the wild-type BGL1A | Phanerodontia chrysosporium |
M177L | site-directed mutagenesis, mutation of the isozyme BGL1A residue from subsite +1 to the correspondent residue of isozyme BGL1B, the mutant shows catalytic efficiency similar to the wild-type BGL1A | Phanerodontia chrysosporium |
V173C | site-directed mutagenesis, mutation of the isozyme BGL1A residue from subsite +1 to the correspondent residue of isozyme BGL1B, the mutant shows catalytic efficiency similar to the wild-type BGL1A | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
cellobiose | pH 6.5, wild-type isozyme BGL1A | Phanerodontia chrysosporium | |
9.22 | - |
cellobiose | pH 6.5, mutant M177L | Phanerodontia chrysosporium | |
13.3 | - |
cellobiose | pH 6.5, mutant V173C | Phanerodontia chrysosporium | |
21 | - |
cellobiose | pH 6.5, mutant H231D | Phanerodontia chrysosporium | |
46.5 | - |
cellobiose | pH 6.5, mutant K253A | Phanerodontia chrysosporium | |
114 | - |
cellobiose | pH 6.5, mutant D229N | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Phanerodontia chrysosporium | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q25BW4 | isozyme BGL1B; gene bgl1B, isozyme BGL1B | - |
Phanerodontia chrysosporium | Q25BW5 | isozyme BGL1A; gene bgl1A, isozyme BGL1A | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
celloheptaose + 6 H2O = 7 beta-D-glucose | the putative acid/base catalyst is Glu170 | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | - |
Phanerodontia chrysosporium | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling and amino acid sequence analysis of isozyme BGL1A, residues V173, M177, D229, H231, K253 are involved in formation of subsite +1 responsible for substrate recognition, overview | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
BGL1A | - |
Phanerodontia chrysosporium |
BGL1B | - |
Phanerodontia chrysosporium |
More | the enzyme belongs to the glycoside hydrolase family 1 | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.53 | - |
cellobiose | pH 6.5, mutant M177L | Phanerodontia chrysosporium | |
3.23 | - |
cellobiose | pH 6.5, mutant D229N | Phanerodontia chrysosporium | |
3.94 | - |
cellobiose | pH 6.5, mutant H231D | Phanerodontia chrysosporium | |
4.35 | - |
cellobiose | pH 6.5, mutant V173C | Phanerodontia chrysosporium | |
4.47 | - |
cellobiose | pH 6.5, mutant K253A | Phanerodontia chrysosporium | |
181 | - |
cellobiose | pH 6.5, wild-type isozyme BGL1A | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
mutant D229N | Phanerodontia chrysosporium |
6 | - |
mutants V173C and D229N/K253A | Phanerodontia chrysosporium |
6.5 | - |
wild-type BGL1A, and mutants M177L, H231D, and K253A | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH-profile of wild-type and mutant isozymes BGL1A, overview | Phanerodontia chrysosporium |
4.5 | 8 | pH-profile of wild-type isozyme BGL1B, overview | Phanerodontia chrysosporium |