Cloned (Comment) | Organism |
---|---|
gene bglb, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli | Thermotoga maritima |
gene cbg1, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview | Agrobacterium tumefaciens |
additional information | construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
0.005 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
0.08 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
0.24 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
2.64 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
18.9 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
42.7 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
gene cbg1 | - |
Agrobacterium tumefaciens B3/73 | - |
gene cbg1 | - |
Thermotoga maritima | - |
gene bglb | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
gene bglb | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography | Agrobacterium tumefaciens |
recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens |
additional information | - |
substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + alpha-L-arabinose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + alpha-L-arabinose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + alpha-L-arabinose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + alpha-L-arabinose | - |
? | |
additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens | ? | - |
? | |
additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima | ? | - |
? | |
additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens B3/73 | ? | - |
? | |
additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycosyl hydrolase family 3 | Agrobacterium tumefaciens |
More | the enzyme belongs to the glycosyl hydrolase family 3 | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Agrobacterium tumefaciens |
85 | - |
- |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
9 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
18.4 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
22.1 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
27.7 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
28.9 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
95.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
119 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | 3.5 | - |
Thermotoga maritima |
6.5 | 8 | - |
Agrobacterium tumefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 11 | - |
Thermotoga maritima |