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Literature summary for 3.2.1.21 extracted from

  • Kwak, S.N.; Kim, S.Y.; Choi, S.R.; Kim, I.S.
    Assembly and function of AsGlu2 fibrillar multimer of oat beta-glucosidase (2009), Biochim. Biophys. Acta, 1794, 526-531.
    View publication on PubMed
Search for more literature for 3.2.1.21

Protein Variants

Protein Variants Comment Organism
E495L isoform AsGlu2. Mutation assembles the AsGlu2 into fibrillar homomultimers. The mutant AsGlu2 homomultimer is highly stable and has relatively faster electric mobility in native gel than the AsGlu1 homomultimer. Multimerization increases enzyme affinity to substrates Avena sativa
additional information swapping analysis of isoforms AsGlu1 and AsGlu2 shows that the C-terminal segment of AsGlu1 is critical for fibrillar multimerization Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 avenacoside pH 6.8, isoform AsGlu1 Avena sativa
0.037
-
 avenacoside pH 6.8, isoform AsGlu2 mutant E495K Avena sativa
0.056
-
 avenacoside pH 6.8, isoform AsGlu2 Avena sativa
4.13
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu1 Avena sativa
5.21
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu2 mutant E495K Avena sativa
10.34
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu2 Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid enzyme beta-glucosidase exists as a long fibrillar structure of isoform AsGlu1 homomultimer, i.e. type I and heteromultimer of isoforms AsGlu1 and AsGlu2, i.e. type II Avena sativa 9536
-

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Avena sativa 4-nitrophenol + D-glucose
-
 ?
avenacoside + H2O
-
 Avena sativa C26-desgluco-avenacosides + D-glucose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu2 mutant E495K Avena sativa
1.2
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu1 Avena sativa
2
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.8, isoform AsGlu2 Avena sativa
4.75
-
 avenacoside pH 6.8, isoform AsGlu2 mutant E495K Avena sativa
402
-
 avenacoside pH 6.8, isoform AsGlu1 Avena sativa
443
-
 avenacoside pH 6.8, isoform AsGlu2 Avena sativa