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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of an intracellular beta-glucosidase from the protoplast fusant of Aspergillus oryzae and Aspergillus niger (2011), Appl. Biochem. Microbiol., 46, 626-632.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glucose | - |
Aspergillus oryzae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
(2-hydroxymethylphenyl)-beta-D-glucopyranoside | pH 4.8, 65°C | Aspergillus oryzae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | completely inhibits enzyme activity at concentration of 10 mM | Aspergillus oryzae | |
| EDTA | completely inhibits enzyme activity at concentration of 10 mM | Aspergillus oryzae | |
| Fe3+ | accelerates enzyme activity | Aspergillus oryzae | |
| K+ | completely inhibits enzyme activity at concentration of 10 mM | Aspergillus oryzae | |
| Mg2+ | completely inhibits enzyme activity at concentration of 10 mM | Aspergillus oryzae | |
| Na+ | completely inhibits enzyme activity at concentration of 10 mM | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
enzyme is purified from the protoplast fusant of Aspergillus oryzae 3.481 and Aspergillus niger 3.316 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 138.85fold by ammonium sulfate precipitation, DE-22 ion exchange and Sephadex G-150 gel filtration chromatography | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
above 75°C, the enzyme shows less than 20% of its optimal activity | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
assay at | Aspergillus oryzae |
| 5.4 | - |
- |
Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.6 | - |
Aspergillus oryzae |
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