Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli under regulation of a T7/lac promoter. On induction with isopropyl-beta-D-1-thiogalactopyranoside, the enzyme expresses at 40% of the cell protein producing 238 mg/liter culture. With increase in culture cell density to A600 12 in auto-inducing M9NG medium, beta-glucosidase production increases 3fold. Approximately 70% of the expressed enzyme is in a soluble form, while the rest is in an insoluble fraction of the cell lysate | Halalkalibacterium halodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 8.0, 45°C | Halalkalibacterium halodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51600 | - |
MALDI-TOF | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation followed by ion-exchange chromatography | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Halalkalibacterium halodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 8.0, 45°C | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Halalkalibacterium halodurans |