Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein PBPI | binding protein, interacts with beta-glucosidases and activates them | Arabidopsis thaliana | |
protein PBPII | binding protein, interacts with beta-glucosidases and activates them | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
cDNA expressed in insect cells, Baculovirus expression system | Arabidopsis thaliana |
cDNA is expressed in insect cells, Baculovirus expression system | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
scopolin | native enzyme, pH 6, 37°C | Arabidopsis thaliana | |
0.81 | - |
scopolin | recombinant enzyme, pH 6, 37°C | Arabidopsis thaliana | |
5.8 | - |
esculin | native enzyme, pH 6, 37°C | Arabidopsis thaliana | |
9.7 | - |
esculin | recombinant enzyme, pH 6, 37°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59700 | - |
BGLU 21, calculated from sequence | Arabidopsis thaliana |
59700 | - |
BGLU 23, calculated from sequence | Arabidopsis thaliana |
59800 | - |
BGLU 22, calculated from seuqence | Arabidopsis thaliana |
63000 | - |
SDS-PAGE, recombinant glycosylated protein | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
scopolin + H2O | Arabidopsis thaliana | - |
scopoletin + beta-D-glucose | - |
? | |
scopolin + H2O | Arabidopsis thaliana | - |
scopoletin + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C525 | - |
- |
Arabidopsis thaliana | Q9C8Y9 | - |
- |
Arabidopsis thaliana | Q9SR37 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | seedling | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-MU-glucoside + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
DIMBOA-glucoside + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
esculin + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
scopolin + H2O | - |
Arabidopsis thaliana | scopoletin + beta-D-glucose | - |
? | |
scopolin + H2O | - |
Arabidopsis thaliana | scopoletin + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Arabidopsis thaliana |
BGLU21 | - |
Arabidopsis thaliana |
BGLU22 | - |
Arabidopsis thaliana |
BGLU23 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
95% activity | Arabidopsis thaliana |
50 | - |
dramatical decrease of activity above 50°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | BGLU22 by cold and mannitol | down |
Arabidopsis thaliana | BGLU23 by NaCl and mannitol | down |
Arabidopsis thaliana | BGLU21 by cold, 2,4-dichlorophenoxyacetate and methyl jasmonate | up |
Arabidopsis thaliana | BGLU22 by NaCl, 2,4-dichlorophenoxyacetate and methyl jasmonate | up |
Arabidopsis thaliana | BGLU23 by 2,4-dichlorophenoxyacetate and methyl jasmonate | up |