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Literature summary for 3.2.1.21 extracted from

  • Kim, B.N.; Yeom, S.J.; Kim, Y.S.; Oh, D.K.
    Characterization of a beta-glucosidase from Sulfolobus solfataricus for isoflavone glycosides (2012), Biotechnol. Lett., 34, 125-129.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 daidzin pH 5.5, 90°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56691
-
 x * 56691, calculated from sequence Saccharolobus solfataricus
57000
-
 x * 57000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
179
-
 pH 5.5, 90°C, substrate: daidzein Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daidzein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
daidzin + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus beta-D-glucose + daidzein
-
 ?
genistein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus beta-D-glucose + genistein
-
 ?
genistin + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus beta-D-glucose + genistein
-
 ?
glycitein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus beta-D-glucose + glycitein
-
 ?
glycitin + H2O the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin Saccharolobus solfataricus beta-D-glucose + glycitein
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Saccharolobus solfataricus
? x * 56691, calculated from sequence Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half-life: 18 h Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2532
-
 daidzin pH 5.5, 90°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5065
-
 daidzin pH 5.5, 90°C Saccharolobus solfataricus