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Literature summary for 3.2.1.21 extracted from

  • Li, D.; Li, X.; Dang, W.; Tran, P.L.; Park, S.H.; Oh, B.C.; Hong, W.S.; Lee, J.S.; Park, K.H.
    Characterization and application of an acidophilic and thermostable beta-glucosidase from Thermofilum pendens (2012), J. Biosci. Bioeng., 115, 490-496.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
ethanol 10%, 1.1fold activation Thermofilum pendens
methanol 10%, 1.1fold activation Thermofilum pendens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermofilum pendens

Inhibitors

Inhibitors Comment Organism Structure
dimethyl sulfoxide 30% 25% inhibition. 40% 47% inhibition. 50% 68% inhibition Thermofilum pendens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.147
-
 4-O-alpha-D-mannopyranosyl-D-mannopyranose pH 3.5, 90°C Thermofilum pendens
0.149
-
 cellobiose pH 3.5, 90°C Thermofilum pendens
0.189
-
 4-nitrophenyl beta-D-glucopyranoside pH 3.5, 90°C Thermofilum pendens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 2fold activation Thermofilum pendens
Cu2+ 5 mM, 1.1fold activation Thermofilum pendens
Mn2+ 5 mM, 1.5fold activation Thermofilum pendens
Zn2+ 5 mM, 1.5fold activation Thermofilum pendens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77800
-
 x * 77800, SDS-PAGE Thermofilum pendens

Organism

Organism UniProt Comment Textmining
Thermofilum pendens A1S0B1
-
-
Thermofilum pendens DSM 2475 A1S0B1
-
-

Purification (Commentary)

Purification (Comment) Organism
Tpen_1494 is ligated into p6xHis119 downstream of the BLMA promoter to create p6xHisTpbgl. Recombinant Tpbgl, with eight additional amino acids (Met-Glu-(His)6) at the N-terminus, is expressed in Escherichia coli harboring p6xHisTpbgl Thermofilum pendens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
 crude extract of recombinant Escherichia coli, pH 3.5, 90°C Thermofilum pendens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O when the enzyme iss incubated with 4-nitrophenyl-beta-D-glucopyranoside in the presence of ethanol and propanol, the glucose moiety is transferred to acceptor alcohols Thermofilum pendens 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O when the enzyme iss incubated with 4-nitrophenyl-beta-D-glucopyranoside in the presence of ethanol and propanol, the glucose moiety is transferred to acceptor alcohols Thermofilum pendens DSM 2475 4-nitrophenol + beta-D-glucose
-
 ?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
-
 Thermofilum pendens 2 D-mannose
-
 ?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
-
 Thermofilum pendens DSM 2475 2 D-mannose
-
 ?
cellobiose + H2O
-
 Thermofilum pendens 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Thermofilum pendens DSM 2475 2 beta-D-glucose
-
 ?
genistin + H2O genistin solubilized in 10-40% dimethylsulfoxide is hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme Thermofilum pendens beta-D-glucose + genistein
-
 ?
genistin + H2O genistin solubilized in 10-40% dimethylsulfoxide is hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme Thermofilum pendens DSM 2475 beta-D-glucose + genistein
-
 ?

Subunits

Subunits Comment Organism
? x * 77800, SDS-PAGE Thermofilum pendens

Synonyms

Synonyms Comment Organism
Tpbgl
-
 Thermofilum pendens
Tpen_1494 locus name Thermofilum pendens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermofilum pendens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 60°C: about 70% of maximal activity, 100°C: about 75% of maxim al activity Thermofilum pendens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 most heat stable under extremely acidic conditions, pH 3.5 Thermofilum pendens
85 90 the enzyme retains nearly 100% of its original activity over 120 min of incubation Thermofilum pendens
90
-
 pH 3.5-4.5, 1 or 2 h, more than 80% of the maximum activity is retained Thermofilum pendens
95
-
 50% activity remains after incubation for 60 min Thermofilum pendens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 4-O-alpha-D-mannopyranosyl-D-mannopyranose pH 3.5, 90°C Thermofilum pendens
0.317
-
 cellobiose pH 3.5, 90°C Thermofilum pendens
0.577
-
 4-nitrophenyl beta-D-glucopyranoside pH 3.5, 90°C Thermofilum pendens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 incubation at 90°C Thermofilum pendens

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3.0: about 50% of maximal activity, pH 5.0: about 80% of maximal activity Thermofilum pendens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 4 90°C, 1 or 2 h, more than 80% of the maximum activity is retained Thermofilum pendens
4.5 6 90°C, 1 or 2 h, 60% of the activity is retained Thermofilum pendens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
 4-O-alpha-D-mannopyranosyl-D-mannopyranose pH 3.5, 90°C Thermofilum pendens
2.13
-
 cellobiose pH 3.5, 90°C Thermofilum pendens
3.05
-
 4-nitrophenyl beta-D-glucopyranoside pH 3.5, 90°C Thermofilum pendens